| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,504,515 | C→T | R267C (CGC→TGC) | insQ → | IS609 transposase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,504,515 | 0 | C | T | 93.5% | 86.5 / ‑1.2 | 31 | R267C (CGC→TGC) | insQ | IS609 transposase B |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (15/14); total (16/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
CAAATATCCGCAGGGACTACCTTCACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTAT > NC_000913/1504384‑1504643 | cAAATATCCGCAGGGACTACCTTCACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGt > 1:283281/1‑139 (MQ=255) aGGGACTACCTTCACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAAt < 2:525265/139‑1 (MQ=255) cacaAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAAcc > 2:789403/1‑139 (MQ=255) cacaAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAAcc > 2:763481/1‑139 (MQ=255) caAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGt > 2:408738/1‑139 (MQ=255) aaaGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGtt < 1:789403/139‑1 (MQ=255) aaGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTc < 2:569488/139‑1 (MQ=255) tCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGa < 1:312296/138‑1 (MQ=255) tCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGa > 2:312296/1‑138 (MQ=255) tCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGAt > 1:586702/1‑139 (MQ=255) cacaACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATa < 1:367940/139‑1 (MQ=255) caACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACt > 2:595909/1‑139 (MQ=255) aCGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTgg > 2:311338/1‑139 (MQ=255) gCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATc > 2:91479/1‑139 (MQ=255) tCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGtt < 1:239272/123‑1 (MQ=255) tCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGtt > 2:239272/1‑123 (MQ=255) tCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAggg < 1:408738/139‑1 (MQ=255) gCAATGATAGTCATTGAGGATTTGAAGGTAAGCAACATGTCAAAGACAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATgc > 1:462950/1‑139 (MQ=255) tCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCt < 1:91479/139‑1 (MQ=255) tGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGtt > 2:185701/1‑72 (MQ=255) tGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGtt < 1:185701/72‑1 (MQ=255) gTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGt < 2:381074/139‑1 (MQ=255) agcagcGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTa < 2:350213/55‑1 (MQ=255) agcagcGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTa > 1:350213/1‑55 (MQ=255) agcagcGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGtgctt < 2:283281/139‑1 (MQ=255) gcagcGGGTACGGTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGtgcttg > 2:126646/1‑139 (MQ=255) ggTCAGCCAGCCGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCa < 1:675723/139‑1 (MQ=255) ggTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCa < 1:311338/139‑1 (MQ=255) ccagccGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAggg < 1:87138/60‑1 (MQ=255) ccagccGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAggg > 2:87138/1‑60 (MQ=255) ccagccGGGGTGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGTGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGtat > 1:308993/1‑139 (MQ=255) | CAAATATCCGCAGGGACTACCTTCACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGCGTATAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTAT > NC_000913/1504384‑1504643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |