Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,995,320	G→A	R14C (CGC→TGC)	uvrY ←	response regulator in two‑component regulatory system with BarA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,995,320	0	G	A	100.0%	134.9 / NA	39	R14C (CGC→TGC)	uvrY	response regulator in two‑component regulatory system with BarA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/23);  total (16/23)

ATGTCCATTAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCGCACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGAAACGTTAAAAATGGT  >  NC_000913/1995196‑1995452                                                                                                                             |                                                                                                                                     aTGTCCATTAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGt                                                                                                                        <  2:453394/139‑1 (MQ=255)      cATTAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGtcatca                                                                                                                   >  1:328762/1‑139 (MQ=255)         taacaccacGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGCTCATcaaca                                                                                                                >  2:513734/4‑139 (MQ=255)         tAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATcaac                                                                                                                 <  1:204198/138‑1 (MQ=255)         tAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATcaac                                                                                                                 >  2:204198/1‑138 (MQ=255)             accacGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTa                                                                                                            <  1:96911/139‑1 (MQ=255)                acGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGaa                                                                                                         >  1:453310/1‑139 (MQ=255)                 cGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATcaac                                                                                                                 >  2:444303/1‑130 (MQ=255)                 cGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATcaac                                                                                                                 <  1:444303/130‑1 (MQ=255)                         gCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCa                                                                                                <  2:570178/139‑1 (MQ=255)                              tGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGa                                                                                           <  2:328762/139‑1 (MQ=255)                                     caccacTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATAtctc                                                                                    <  1:439120/139‑1 (MQ=255)                                       ccacTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCa                                                                                  <  1:350892/139‑1 (MQ=255)                                           ttAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGaaa                                                                              <  2:92245/139‑1 (MQ=255)                                                   gTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATa                                                                      <  1:438544/139‑1 (MQ=255)                                                            gCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTg                                                             <  1:15182/139‑1 (MQ=255)                                                                  tGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagtt                                                       >  2:693883/1‑139 (MQ=255)                                                                  tGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagtt                                                       >  1:64929/1‑139 (MQ=255)                                                                  tGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagtt                                                       >  1:279567/1‑139 (MQ=255)                                                                     cTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagttagt                                                    <  1:264322/139‑1 (MQ=255)                                                                     cTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagttagt                                                    <  1:229404/139‑1 (MQ=255)                                                                        aCCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCAt                                                 <  1:513734/139‑1 (MQ=255)                                                                        aCCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCAt                                                 <  1:794133/139‑1 (MQ=255)                                                                                                   ttCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGa                                                                                           <  1:270269/70‑1 (MQ=255)                                                                                                   ttCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGa                                                                                           >  2:270269/1‑70 (MQ=255)                                                                                                   ttCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACt                                                              <  1:479366/99‑1 (MQ=255)                                                                                                   ttCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACt                                                              >  2:479366/1‑99 (MQ=255)                                                                                                    tCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCAt                                                 <  1:318002/111‑1 (MQ=255)                                                                                                    tCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCAt                                                 >  2:318002/1‑111 (MQ=255)                                                                                                     ccAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGt                                                                                                                        <  2:92730/39‑1 (MQ=255)                                                                                                     ccAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGt                                                                                                                        >  1:92730/1‑39 (MQ=255)                                                                                                     ccAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagtta                                                      >  2:573207/1‑105 (MQ=255)                                                                                                     ccAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagt                                                        <  1:88955/103‑1 (MQ=255)                                                                                                     ccAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagt                                                        >  2:88955/1‑103 (MQ=255)                                                                                                     ccAGAATGCGTCGTATCCCTGCGCACACAAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagtta                                                      <  1:573207/105‑1 (MQ=255)                                                                                                        gAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGa                 <  2:453310/139‑1 (MQ=255)                                                                                                         aaTGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGaa                >  1:30627/1‑139 (MQ=255)                                                                                                             cgtcgtATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGAAACGt            >  1:11212/1‑139 (MQ=255)                                                                                                                       cTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGAAACGTTAAAAAtggt  <  1:693883/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                     ATGTCCATTAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCGCACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGAAACGTTAAAAATGGT  >  NC_000913/1995196‑1995452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑