Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,073,094 T→C L519P (CTG→CCG)  flu → CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,073,0940TC98.3% 425.1 / ‑2.7 115L519P (CTG→CCG) fluCP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (54/59);  total (55/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01

Only 100 of 135 total aligned reads displayed.

ATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAA  >  NC_000913/2072958‑2073229
                                                                                                                                        |                                                                                                                                       
aTCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccga                                                                                                                                       >  1:441103/1‑139 (MQ=255)
  ccGTGAAGGCGATGCACTGCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacg                                                                                                                                     >  2:528514/1‑139 (MQ=255)
  ccGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacg                                                                                                                                     >  1:223661/1‑139 (MQ=255)
   cGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgc                                                                                                                                    <  2:793259/139‑1 (MQ=255)
    gTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgct                                                                                                                                   >  1:448687/1‑139 (MQ=255)
     tGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                  <  1:250563/139‑1 (MQ=255)
       aaGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGaa                                                                                                                                <  1:33731/139‑1 (MQ=255)
            gATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACa                                                                                                                           >  1:786307/1‑139 (MQ=255)
             aTGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAg                                                                                                                          >  2:752368/1‑139 (MQ=255)
             aTGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAg                                                                                                                          >  1:99342/1‑139 (MQ=255)
               gCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTa                                                                                                                        >  1:279346/1‑139 (MQ=255)
                    ccTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTc                                                                                                                   >  2:647169/1‑139 (MQ=255)
                     cTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccga                                                                                                                                       >  1:420778/1‑118 (MQ=255)
                         aGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcaccac                                                                                                              <  1:799611/139‑1 (MQ=255)
                          gggAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACg                                                                                                             <  1:207994/139‑1 (MQ=255)
                             aGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTca                                                                                                                  <  2:697588/131‑1 (MQ=255)
                               gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                  >  1:114031/1‑113 (MQ=255)
                               gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcacca                                                                                                               >  2:365794/1‑132 (MQ=255)
                               gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTcacca                                                                                                               <  1:365794/132‑1 (MQ=255)
                               gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGa                                                                                                           >  2:285455/1‑136 (MQ=255)
                               gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGa                                                                                                           <  1:285455/136‑1 (MQ=255)
                               gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGt                                                                                                        >  1:66766/1‑139 (MQ=255)
                               gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGt                                                                                                        >  1:178867/1‑139 (MQ=255)
                               gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGt                                                                                                        >  2:775949/1‑139 (MQ=255)
                                  ctctcACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGa                                                                                                                             <  1:534890/115‑1 (MQ=255)
                                      cACCGGTAACGGCAGCGTGGAAAAATCAGGAGGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                                                 <  2:206272/139‑1 (MQ=255)
                                      cACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                                                 <  2:597933/139‑1 (MQ=255)
                                      cACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTAACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                                                 <  1:655738/139‑1 (MQ=255)
                                         cGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTATCGTCACCACGGATGTCATTGCTCAg                                                                                              >  2:246990/1‑139 (MQ=255)
                                         cGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAg                                                                                              >  1:619295/1‑139 (MQ=255)
                                           gTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                            <  2:604986/139‑1 (MQ=255)
                                           gTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                            <  2:186994/139‑1 (MQ=255)
                                           gTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                            <  2:114413/139‑1 (MQ=255)
                                           gTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                            <  1:192551/139‑1 (MQ=255)
                                               cGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGt                                                                                        >  2:53389/1‑139 (MQ=255)
                                                 gCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACGGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTAc                                                                                      <  1:192457/139‑1 (MQ=255)
                                                 gCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGa                                                                                                           <  2:567959/118‑1 (MQ=255)
                                                 gCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGa                                                                                                           >  1:567959/1‑118 (MQ=255)
                                                 gCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGa                                                                                                           <  1:495551/118‑1 (MQ=255)
                                                 gCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGa                                                                                                           >  2:495551/1‑118 (MQ=255)
                                                     cGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGcc                                                                                  >  2:742509/1‑139 (MQ=255)
                                                     cGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGcc                                                                                  >  1:148345/1‑139 (MQ=255)
                                                              aTCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTg                                                                         <  2:260864/139‑1 (MQ=255)
                                                                cAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGAc                                                                       <  2:191497/139‑1 (MQ=255)
                                                                   gAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCgg                                                                    >  1:151081/1‑139 (MQ=255)
                                                                     aGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgca                                                                  <  1:775949/139‑1 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACg                                                                                                             <  2:304901/93‑1 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               <  1:53389/139‑1 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               >  2:42440/1‑139 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               <  1:654730/139‑1 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               <  2:659298/139‑1 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGGACAGCCGTGAAGCTGACCGacagcg                                                               <  1:618822/139‑2 (MQ=255)
                                                                        ggCACACTCACTGTCAGCAACACCACACTCACCCACAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               <  2:769659/139‑1 (MQ=255)
                                                                         gCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCAc                                                              >  1:21107/1‑139 (MQ=255)
                                                                             actcactGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACtgtg                                                          <  2:786307/139‑1 (MQ=255)
                                                                                  ctGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGaa                                                     <  2:178867/139‑1 (MQ=255)
                                                                                  ctGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGaa                                                     <  2:467272/139‑1 (MQ=255)
                                                                                   tGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGTAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgca                                                                  >  1:566643/1‑125 (MQ=255)
                                                                                   tGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               >  2:674242/1‑128 (MQ=255)
                                                                                   tGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               <  1:674242/128‑1 (MQ=255)
                                                                                   tGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgca                                                                  <  2:566643/125‑1 (MQ=255)
                                                                                   tGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAAc                                                    >  1:705660/1‑139 (MQ=255)
                                                                                   tGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAAc                                                    >  2:765213/1‑139 (MQ=255)
                                                                                       aGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTg                                                >  2:103109/1‑139 (MQ=255)
                                                                                         cAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGcc                                              >  2:776965/1‑139 (MQ=255)
                                                                                         cAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGcc                                              >  1:453017/1‑139 (MQ=255)
                                                                                           acacCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                                            >  2:39218/1‑91 (MQ=255)
                                                                                             accacACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               >  2:603947/1‑118 (MQ=255)
                                                                                              ccacACTCACCCAGAAAGCCGTCTACCTGAATGAAGGCACGCCGACGCTGAACGGCAGTACCGTCACCACGGATGTCATCGCTCTGCGCGGTACAGCCCAGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGa                                         <  1:528514/139‑1 (MQ=255)
                                                                                               cacaCTCACCCAGAAAGCCGTCAACCTGAATGAAGGCacgccgacgctg                                                                                                                                  >  2:802130/1‑49 (MQ=255)
                                                                                               cacaCTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGAc                                        <  2:223661/139‑1 (MQ=255)
                                                                                               cacaCTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGACGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGAc                                        <  1:752368/139‑1 (MQ=255)
                                                                                                    tCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCAc                                   >  2:34340/1‑139 (MQ=255)
                                                                                                      aCCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTg                                                                         <  1:511012/99‑1 (MQ=255)
                                                                                                      aCCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTg                                                                         >  2:511012/1‑99 (MQ=255)
                                                                                                      aCCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCa                                    >  1:479051/1‑136 (MQ=255)
                                                                                                      aCCCAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGa                                 >  2:11449/1‑139 (MQ=255)
                                                                                                        ccAGAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAAt                               >  1:438487/1‑139 (MQ=255)
                                                                                                           gAAAGCCGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTc                            >  2:60903/1‑139 (MQ=255)
                                                                                                                ccGTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCActct                       <  1:731837/139‑1 (MQ=255)
                                                                                                                  gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCg                     >  2:259599/1‑139 (MQ=255)
                                                                                                                  gTCAACCTGAATGAAGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCg                     >  1:209125/1‑139 (MQ=255)
                                                                                                                          gaatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCAc                                   >  1:603886/1‑117 (MQ=255)
                                                                                                                           aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCGCGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg            <  2:487009/139‑1 (MQ=255)
                                                                                                                           aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTg                                                                         >  2:241280/1‑78 (MQ=255)
                                                                                                                           aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTg                                                                         <  1:241280/78‑1 (MQ=255)
                                                                                                                           aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg            <  1:469694/139‑1 (MQ=255)
                                                                                                                           aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg            <  1:345226/139‑1 (MQ=255)
                                                                                                                           aatgaaGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTg            <  2:705660/139‑1 (MQ=255)
                                                                                                                                aGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               >  1:441276/1‑83 (MQ=255)
                                                                                                                                aGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                                               <  2:441276/83‑1 (MQ=255)
                                                                                                                                aGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGt             >  2:439036/1‑133 (MQ=255)
                                                                                                                                aGGCACGCCGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCAcc       <  2:148345/139‑1 (MQ=255)
                                                                                                                                    acgccgacgctgAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGa   <  1:82549/139‑1 (MQ=255)
                                                                                                                                    acgccgacgctgAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGa   <  1:666702/139‑1 (MQ=255)
                                                                                                                                    acgccgacgctgAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGa   <  2:279308/139‑1 (MQ=255)
                                                                                                                                    acgccgacgctgAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGa   <  1:60903/139‑1 (MQ=255)
                                                                                                                                    acgccgacgctgAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGa   <  2:21107/139‑1 (MQ=255)
                                                                                                                                     cgccgacgctgaACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTc                 <  1:490578/124‑1 (MQ=255)
                                                                                                                                     cgccgacgctgaACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGaa  <  1:274901/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
ATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAA  >  NC_000913/2072958‑2073229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: