Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,166,540 T→C R291R (CGT→CGC yegQ → putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,166,5400TC100.0% 110.7 / NA 30R291R (CGT→CGCyegQputative peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/17);  total (13/17)

CGTCCGGGCGAGTATATGACCGCGTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTGCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCTATA  >  NC_000913/2166406‑2166670
                                                                                                                                      |                                                                                                                                  
cGTCCGGGCGAGTATATGACCGCGTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCa                                                                                                                                <  2:586851/139‑1 (MQ=255)
   ccGGGCGAGTATATGACCGCGTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAAt                                                                                                                             <  1:570459/139‑1 (MQ=255)
   ccGGGCGAGTATATGACCGCGTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAAt                                                                                                                             <  1:636721/139‑1 (MQ=255)
   ccGGGCGAGTATATGACCGCGTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAAt                                                                                                                             <  1:771069/139‑1 (MQ=255)
   ccGGGCGAGTATATGACCGCGTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAAt                                                                                                                             <  2:100683/139‑1 (MQ=255)
   ccGGGCGAGTATATGACCGCGTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAAt                                                                                                                             <  2:515897/139‑1 (MQ=255)
     gggCGAGTATATGACCGCGTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCt                                                                                                                           <  2:135265/139‑1 (MQ=255)
     gggCGAGTATATGACCGCGTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCt                                                                                                                           <  1:768428/139‑1 (MQ=255)
                      cgTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTcta                                                                                                                      >  2:578833/1‑127 (MQ=255)
                      cgTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTcta                                                                                                                      <  1:578833/127‑1 (MQ=255)
                      cgTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTgcacg                                                                                                          >  1:672499/1‑139 (MQ=255)
                                  aGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCACGCACCGCACAGGt                                                                                              <  2:175526/139‑1 (MQ=255)
                                      cGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCACGCACCGCACAGGTTTAc                                                                                          >  2:513766/1‑139 (MQ=255)
                                          aCTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCACGCACCGCACAGGTTTACCGCa                                                                                      <  2:750808/139‑1 (MQ=255)
                                                             aaGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCACGCACCGCACAg                                                                                                <  1:785900/110‑1 (MQ=255)
                                                             aaGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCACGCACCGCACAg                                                                                                >  2:785900/1‑110 (MQ=255)
                                                                     cgcgcCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCgggca                                                           >  1:776442/1‑137 (MQ=255)
                                                                                          cGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTgcacgcac                                                                                                       >  1:396681/1‑74 (MQ=255)
                                                                                          cGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTgcacgcac                                                                                                       <  2:396681/74‑1 (MQ=255)
                                                                                             cTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCAcgcaccgca                                                                                                   >  2:451660/1‑75 (MQ=255)
                                                                                             cTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCAcgcaccgca                                                                                                   <  1:451660/75‑1 (MQ=255)
                                                                                             cTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCAcgcaccg                                                                                                     >  1:797268/1‑73 (MQ=255)
                                                                                             cTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCAcgcaccg                                                                                                     <  2:797268/73‑1 (MQ=255)
                                                                                             cTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTgg                                   >  2:649851/1‑139 (MQ=255)
                                                                                              tGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGa                                  <  2:411042/139‑1 (MQ=255)
                                                                                              tGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGa                                  >  1:411042/1‑139 (MQ=255)
                                                                                                          gCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCAcgcaccgca                                                                                                   <  2:260905/62‑1 (MQ=255)
                                                                                                          gCGTGCATTCGCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCAcgcaccgca                                                                                                   >  1:260905/1‑62 (MQ=255)
                                                                                                                    gCTGAAAATCGAAGGTCGCACCAAATCTTTCTACTATTGTGCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCAt            >  1:538506/1‑139 (MQ=255)
                                                                                                                              gAAGGTCGCACCAAATCTTTCTACTATTGTGCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCtata  >  2:587207/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                  
CGTCCGGGCGAGTATATGACCGCGTTTGAAGATGAGCACGGCACTTACATCATGAACTCGAAAGATCTGCGCGCCATCGCCCATGTAGAACGCCTGACCAAAATGGGCGTGCATTCGCTGAAAATCGAAGGTCGTACCAAATCTTTCTACTATTGTGCACGCACCGCACAGGTTTACCGCAAAGCTATCGATGACGCCGCTGCGGGAAAACCGTTCGATACCAGCCTGCTGGAAACTCTGGAAGGTCTGGCGCATCGTGGCTATA  >  NC_000913/2166406‑2166670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: