| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,237,322 | G→A | I144I (ATC→ATT) | mglC ← | methyl‑galactoside transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,237,322 | 0 | G | A | 91.9% | 101.7 / 1.7 | 37 | I144I (ATC→ATT) | mglC | methyl‑galactoside transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (20/14); total (21/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 | |||||||||||
ATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCA > NC_000913/2237197‑2237455 | aTGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGc > 1:153476/1‑139 (MQ=255) gagagaCGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCg < 2:630432/139‑1 (MQ=255) aCGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTa > 2:277566/1‑139 (MQ=255) cGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTaa > 2:395555/1‑139 (MQ=255) cTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACg < 2:153476/139‑1 (MQ=255) aGCAAAGGTAGAGAAGCCACTGTCAGAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATa > 2:307695/1‑139 (MQ=255) gagaAGCCACTGTCAAAACCAGAAATTGGCGACGCGCCGACAAAGGCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCg > 1:77238/1‑94 (MQ=255) gagaAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCg < 2:77238/94‑1 (MQ=255) cACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCaa > 2:635162/1‑139 (MQ=255) aCTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCg < 2:662353/101‑1 (MQ=255) aCTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCg > 1:662353/1‑101 (MQ=255) aaaTTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCAcc > 2:141012/1‑139 (MQ=255) aaaTTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCAcc > 2:455929/1‑139 (MQ=255) aaaTTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCAcc > 1:184836/1‑139 (MQ=255) ggCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATgg < 1:277566/139‑1 (MQ=255) cGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCa < 1:656748/104‑1 (MQ=255) cGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCa > 2:656748/1‑104 (MQ=255) cGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGca < 1:635162/139‑1 (MQ=255) aCGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGcaca < 1:120432/139‑1 (MQ=255) ccGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACaa < 1:141012/139‑1 (MQ=255) ccGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACaa < 1:455929/139‑1 (MQ=255) ccGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACaa < 2:184836/139‑1 (MQ=255) tAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAgcg < 2:79148/139‑1 (MQ=255) gCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGca < 1:395159/113‑1 (MQ=255) gCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGca > 2:395159/1‑113 (MQ=255) gCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAAt > 2:593436/1‑139 (MQ=255) gCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAAt > 1:239681/1‑139 (MQ=255) gCGAGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAAt > 2:420137/1‑139 (MQ=255) aGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGa < 1:625622/114‑1 (MQ=255) aGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGa > 2:625622/1‑114 (MQ=255) aGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAg < 1:209503/130‑1 (MQ=255) aGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAg > 2:209503/1‑130 (MQ=255) aGTTGATGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCgg > 1:694384/1‑139 (MQ=255) tGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATgg > 2:174685/1‑102 (MQ=255) tGCCATAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATgg < 1:174685/102‑1 (MQ=255) tAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCa > 1:97383/1‑139 (MQ=255) tAGACAATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCa > 2:683208/1‑139 (MQ=255) | ATGTAAGAGAGACGGAAACTCCCCAGCGCGACAAAGCCCTGAGCAAAGGTAGAGAAGCCACTGTCAAAACCAGAAATTGGCGACGCCCCGACAAAGTCGTAATAGAGCGAGTTGATGCCATAGACGATGATCATCGTGCCGAGCGTGGTAATGAACGGCGTCACGTTGAGATAAGCGATAATCAGACCGTTGATCAAACCGATCACCGCACCAATGGCACAGACAATCAGAATAACCAGCGCAATCGGCATCGTCGCCA > NC_000913/2237197‑2237455 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |