Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	290,698	T→A	Q152L (CAG→CTG)	insB1 ←	IS1 transposase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	290,698	0	T	A	85.7%	12.7 / ‑2.5	7	Q152L (CAG→CTG)	insB1	IS1 transposase B
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (3/3);  total (4/3)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01

GGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGAT  >  NC_000913/290563‑290761                                                                                                                                        |                                                                ggAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATgcagct                                                              >  1:24131/1‑139 (MQ=255)                     ctgcCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGAc                                          >  1:389217/1‑139 (MQ=255)                        ccTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGc                                       <  2:24131/139‑1 (MQ=255)                          ttacttcattacttcaGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGa                                     >  1:293991/1‑139 (MQ=255)                                        caGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGcc                       >  1:450721/1‑139 (MQ=255)                                        caGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGcc                       >  2:357156/1‑139 (MQ=255)                                        caGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGcc                       <  2:389217/139‑1 (MQ=255)                                                             atCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGATCCACCGATTTTGAGAACGACAGCTACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGAt  <  1:293985/139‑1 (MQ=255)                                                                                                                                        |                                                                GGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGAT  >  NC_000913/290563‑290761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑