Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,277,291	C→T	A331V (GCG→GTG)	yejF →	microcin C ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,277,291	0	C	T	100.0%	81.5 / NA	25	A331V (GCG→GTG)	yejF	microcin C ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/10);  total (15/10)

TCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATT  >  NC_000913/2277160‑2277402                                                                                                                                    |                                                                                                                tCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGctgctg                                                                                                          >  1:387862/1‑139 (MQ=255)       aGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGATTTCCGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTg                                                                                                    >  1:50024/1‑139 (MQ=255)                gAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATtct                                                                                           <  2:50024/139‑1 (MQ=255)                   gcgcATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGGTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAg                                                                                        >  1:256971/1‑139 (MQ=255)                      cATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGgc                                                                                     >  2:93292/1‑139 (MQ=255)                                     tgtgGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGtgctgc                                                                                                            <  1:264604/101‑1 (MQ=255)                                     tgtgGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGtgctgc                                                                                                            >  2:264604/1‑101 (MQ=255)                                     tgtgGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGAc                                                                      >  1:238940/1‑139 (MQ=255)                                         gtggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa                                                                       >  2:20954/1‑134 (MQ=255)                                         gtggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa                                                                       <  1:20954/134‑1 (MQ=255)                                         gtggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTc                                                                  >  1:699153/1‑139 (MQ=255)                                          tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGgcagca                                                                                 >  1:455518/1‑123 (MQ=255)                                          tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGgcagca                                                                                 <  2:455518/123‑1 (MQ=255)                                                         gTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAAttt                                                  >  2:413344/1‑139 (MQ=255)                                                               cGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATcg                                            >  1:271745/1‑139 (MQ=255)                                                                   aCGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATcgccgc                                        <  1:215949/139‑1 (MQ=255)                                                                    cGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTTAGTGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCgccgcc                                       >  1:319787/1‑139 (MQ=255)                                                                      aGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAg                                     <  2:52108/139‑1 (MQ=255)                                                                          ggTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGgcag                                                                                   <  1:304308/89‑1 (MQ=255)                                                                          ggTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGgcag                                                                                   >  2:304308/1‑89 (MQ=255)                                                                          ggTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCGACTGCAAAATTTAAATCGCCGCCAGCTGt                                 >  1:516935/1‑139 (MQ=255)                                                                           gTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGtt                                <  2:238940/139‑1 (MQ=255)                                                                                        tttAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCAt                   <  2:699153/139‑1 (MQ=255)                                                                                              gggCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCAtt             <  2:387862/139‑1 (MQ=255)                                                                                                gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCa           <  1:667331/139‑1 (MQ=255)                                                                                                gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCa           >  1:7132/1‑139 (MQ=255)                                                                                                         gTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTAtt  >  2:301880/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                TCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATT  >  NC_000913/2277160‑2277402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑