Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,314,922 T→C S479P (TCC→CCC)  rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,314,9220TC100.0% 101.6 / NA 27S479P (TCC→CCC) rcsDphosphotransfer intermediate protein in two‑component regulatory system with RcsBC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/15);  total (12/15)

CATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGA  >  NC_000913/2314786‑2315057
                                                                                                                                        |                                                                                                                                       
cATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGccc                                                                                                                                       <  1:212030/139‑1 (MQ=255)
                gATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGCAAAACCAGCAGGGGCGGATGATCTTTATGCAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCgg                                                                                                                       <  1:527942/139‑1 (MQ=255)
                                                  aGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGc                                                                                     <  2:269963/139‑1 (MQ=255)
                                                              tGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGa                                                                         >  1:37785/1‑139 (MQ=255)
                                                                tATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGa                                                                         >  2:758700/1‑137 (MQ=255)
                                                                tATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGa                                                                         <  1:758700/137‑1 (MQ=255)
                                                                      aaaaaCCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAgcg                                                                                                                          <  2:664626/82‑1 (MQ=255)
                                                                      aaaaaCCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAgcg                                                                                                                          >  1:664626/1‑82 (MQ=255)
                                                                          aCCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCg                                                             >  2:584003/1‑139 (MQ=255)
                                                                          aCCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCg                                                             >  1:482188/1‑139 (MQ=255)
                                                                          aCCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCg                                                             >  2:268985/1‑139 (MQ=255)
                                                                          aCCAGCAGGGGCGGATAATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCg                                                             >  2:749177/1‑139 (MQ=255)
                                                                           ccAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCgg                                                            <  2:482188/139‑1 (MQ=255)
                                                                                agGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGt                                                       <  2:100044/139‑1 (MQ=255)
                                                                                    gCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTtgat                                                   <  1:268985/139‑1 (MQ=255)
                                                                                         tgatCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAATGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAAt                                              >  2:728170/1‑139 (MQ=255)
                                                                                              tttATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGt                                         >  1:205547/1‑139 (MQ=255)
                                                                                                  tGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGc                                     >  2:116893/1‑139 (MQ=255)
                                                                                                      aaaCATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAAc                                 <  1:736947/139‑1 (MQ=255)
                                                                                                      aaaCATTGGCGATGCGCTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAAc                                 >  2:736947/1‑139 (MQ=255)
                                                                                                                   gcgcTGAAAGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGgatg                    <  2:196223/139‑1 (MQ=255)
                                                                                                                          aaGAACCCGCACAGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTg             <  1:235549/139‑1 (MQ=255)
                                                                                                                                    acaGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGaaa                                               <  2:120985/95‑1 (MQ=255)
                                                                                                                                    acaGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGaaa                                               >  1:120985/1‑95 (MQ=255)
                                                                                                                                     caGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga  <  1:116893/139‑1 (MQ=255)
                                                                                                                                     caGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga  <  1:75919/139‑1 (MQ=255)
                                                                                                                                     caGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga  <  2:626759/139‑1 (MQ=255)
                                                                                                                                     caGCCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTAGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTgaga  <  1:749177/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGA  >  NC_000913/2314786‑2315057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: