Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,380,852	T→C	D59G (GAC→GGC)	elaB ←	putative membrane‑anchored DUF883 family ribosome‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,380,852	0	T	C	100.0%	122.4 / NA	33	D59G (GAC→GGC)	elaB	putative membrane‑anchored DUF883 family ribosome‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/14);  total (19/14)

TTTAACGGCGTGCCAGCAACAGTCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGTCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTG  >  NC_000913/2380721‑2380969                                                                                                                                    |                                                                                                                      tttAACGGCGTGCCAGCAACAGTCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGc                                                                                                                <  2:90665/139‑1 (MQ=255)         cGTGCCAGCAACAGTCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTaa                                                                                                        >  1:396253/1‑139 (MQ=255)          gTGCCAGCAACAGTCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAAc                                                                                                       >  2:777862/1‑139 (MQ=255)                       tCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTcaca                                                                                          >  1:604931/1‑139 (MQ=255)                               ccAGCCCAACGGCTGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAg                                                                                  <  1:209864/139‑1 (MQ=255)                                 aGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTCTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAgcg                                                                                >  2:296834/1‑139 (MQ=255)                                   cccAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCt                                                                              >  2:34969/1‑139 (MQ=255)                                       aCGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCttttt                                                                          >  1:300134/1‑139 (MQ=255)                                              cgcCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCAcg                                                                   <  2:396253/139‑1 (MQ=255)                                                         aTTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCt                                                        >  2:122167/1‑139 (MQ=255)                                                             cTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAAc                                                    <  1:777862/139‑1 (MQ=255)                                                               tGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACat                                                  >  1:661269/1‑139 (MQ=255)                                                               tGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACat                                                  >  1:726542/1‑139 (MQ=255)                                                                 ccAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACatat                                                >  1:662130/1‑139 (MQ=255)                                                                             gTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATcggcg                                    <  2:300134/139‑1 (MQ=255)                                                                                   gTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCg                              >  2:185414/1‑139 (MQ=255)                                                                                           caGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATAGTTCTGATCGGCGGGATCGCCAgagga                      <  2:604931/139‑1 (MQ=255)                                                                                            aGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAgaggag                     <  2:320501/139‑1 (MQ=255)                                                                                              cACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCg                                                                                                    <  1:206322/58‑1 (MQ=255)                                                                                              cACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCg                                                                                                    >  2:206322/1‑58 (MQ=255)                                                                                              cACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATcggc                                     <  2:29949/121‑1 (MQ=255)                                                                                              cACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATcggc                                     >  1:29949/1‑121 (MQ=255)                                                                                                 gATAAACTGCCTGCTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAggagcggag                >  1:450604/1‑139 (MQ=255)                                                                                                        tgcctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACctct         >  1:753713/1‑139 (MQ=255)                                                                                                        tgcctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACctct         >  1:150373/1‑139 (MQ=255)                                                                                                          cctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTc       <  1:88847/139‑1 (MQ=255)                                                                                                          cctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTc       <  2:661269/139‑1 (MQ=255)                                                                                                           ctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCt                                                        >  2:274550/1‑89 (MQ=255)                                                                                                           ctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCt                                                        <  1:274550/89‑1 (MQ=255)                                                                                                           ctgcTTCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTcc      >  1:41932/1‑139 (MQ=255)                                                                                                               ttCGCCCGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAgtg  <  2:41932/139‑1 (MQ=255)                                                                                                                   cccGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAGCATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTcc      >  2:426695/1‑131 (MQ=255)                                                                                                                   cccGATAGTAATAACTGCCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTcc      <  1:426695/131‑1 (MQ=255)                                                                                                                                    |                                                                                                                      TTTAACGGCGTGCCAGCAACAGTCCTAGTACCAGCCCAACGGCCGCGCCCACACCAATTCCTTGCCAGGGTTTTTCGTGGACGTAGTCATCAGCACGATAAACTGCCTGCTTCGCCCGATAGTAATAACTGTCTGAAGCCTGGCTAACCCGTTTTTTCACATCATCCAGCGCTTTTTCTGCACGCGCTTTCAGCTCAACATATTTCTGATCGGCGGGATCGCCAGAGGAGCGGAGCACCTCTTCCAGTG  >  NC_000913/2380721‑2380969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑