Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,829,493	G→A	S149S (TCG→TCA)	srlR →	sorbitol‑inducible srl operon transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,829,493	0	G	A	100.0%	123.3 / NA	37	S149S (TCG→TCA)	srlR	sorbitol‑inducible srl operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/20);  total (17/20)

GCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCA  >  NC_000913/2829373‑2829628                                                                                                                         |                                                                                                                                        gCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCaa                                                                                                                       <  1:611858/139‑1 (MQ=255)  ctctCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCAc                                                                                                                             <  1:177326/132‑1 (MQ=255)  ctctCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCAc                                                                                                                             >  2:177326/1‑132 (MQ=255)            ttAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGaa                                                                                                            >  1:253486/1‑139 (MQ=255)                   cATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTc                                                                                                     <  1:623745/139‑1 (MQ=255)                    aTCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACTAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTGCACGGGCAACTGGCAGAGAATGCCTTCg                                                                                                    <  1:582560/139‑1 (MQ=255)                     tCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGa                                                                                                   >  1:56466/1‑139 (MQ=255)                      cACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAg                                                                                                  <  2:253486/139‑1 (MQ=255)                       aCGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCg                                                                                                    >  2:278741/1‑136 (MQ=255)                       aCGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCg                                                                                                    <  1:278741/136‑1 (MQ=255)                                aCCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCg                                                                                                    >  1:515708/1‑127 (MQ=255)                                aCCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCg                                                                                                    <  2:515708/127‑1 (MQ=255)                                     cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCg                                                                                                    >  1:489578/1‑122 (MQ=255)                                     cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCg                                                                                                    <  2:489578/122‑1 (MQ=255)                                     cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGAt                                                                                   >  2:508463/1‑139 (MQ=255)                                     cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGAt                                                                                   >  1:32565/1‑139 (MQ=255)                                      aGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCTAGCATTTCACCTTCGATa                                                                                  <  2:527577/139‑1 (MQ=255)                                        ccTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATaaa                                                                                <  1:85873/139‑1 (MQ=255)                                         cTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAAt                                                                               >  1:669474/1‑139 (MQ=255)                                         cTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAAt                                                                               <  2:32565/139‑1 (MQ=255)                                          tGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATaaact                                                                              >  2:286400/1‑137 (MQ=255)                                                  gTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCt                                                                                        <  1:255921/121‑1 (MQ=255)                                                  gTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCt                                                                                        >  2:255921/1‑121 (MQ=255)                                                      aTGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCAc                                                                  >  2:96313/1‑139 (MQ=255)                                                           cTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCaa                                                                                                                       <  1:536151/81‑1 (MQ=255)                                                           cTATCCGAACTGGATAACGAACAAACTATCCTGATGCAAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCaa                                                                                                                       >  2:536151/1‑81 (MQ=255)                                                            tATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACgg                                                            >  2:35535/1‑139 (MQ=255)                                                              tCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCa                                                          >  1:598234/1‑139 (MQ=255)                                                                        aTAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCaa                                                >  1:768410/1‑139 (MQ=255)                                                                        aTAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCaa                                                >  1:145788/1‑139 (MQ=255)                                                                            cGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCg                                            <  2:768410/139‑1 (MQ=255)                                                                             gAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCgg                                           <  1:96313/139‑1 (MQ=255)                                                                                    cTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAac                                    <  2:617151/139‑1 (MQ=255)                                                                                              gCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAAc                          <  2:669474/139‑1 (MQ=255)                                                                                              gCCAGGCGGAACGTTTCGCAAAAAATCAGCCTCATTTCACAGACATCTGCCAGTGAATGCCTTCGATCATTTCACCTGCGAGAAATTGTTTATGGGCAGCGACGGGATCGATCTTAATGCGGGCGTAACCACCTTTAAc                          <  1:142632/139‑1 (MQ=255)                                                                                                                      aTCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCa  <  1:24675/139‑1 (MQ=255)                                                                                                                      aTCAGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCa  <  2:56466/139‑1 (MQ=255)                                                                                                                         |                                                                                                                                        GCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCA  >  NC_000913/2829373‑2829628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑