| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,834,077 | G→A | A56T (GCC→ACC) | norW → | NADH:flavorubredoxin oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,834,077 | 0 | G | A | 90.3% | 83.5 / 2.2 | 31 | A56T (GCC→ACC) | norW | NADH:flavorubredoxin oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (16/12); total (17/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
GCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACG > NC_000913/2833943‑2834212 | gCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGa < 1:510652/139‑1 (MQ=255)gCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGa < 1:785108/139‑1 (MQ=255) ttCGCAAACAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAAtt > 2:34464/1‑139 (MQ=255) aCAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAg < 1:387534/139‑1 (MQ=255) aCTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATc > 2:417701/1‑139 (MQ=255) cTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCt < 1:417701/139‑1 (MQ=255) ccTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAAt < 1:28550/124‑1 (MQ=255) ccTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAAt > 2:28550/1‑124 (MQ=255) tGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCcaca < 1:531502/139‑1 (MQ=255) gATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTg > 2:596518/1‑130 (MQ=255) gATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTg < 1:596518/130‑1 (MQ=255) ttGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAAc < 1:458494/139‑1 (MQ=255) cATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGat > 2:101153/1‑139 (MQ=255) tGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGatat > 2:798552/1‑139 (MQ=255) aGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGc > 1:64431/1‑139 (MQ=255) aaaCCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGccc < 1:34464/139‑1 (MQ=255) aCCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCcaca < 1:10841/100‑1 (MQ=255) aCCTCAGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCcaca > 2:10841/1‑100 (MQ=255) aGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCg < 2:228059/115‑1 (MQ=255) aGCCATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCg > 1:228059/1‑115 (MQ=255) ccATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTg < 1:529378/103‑1 (MQ=255) ccATGTTATCAGTCAGGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTg > 2:529378/1‑103 (MQ=255) agGGGCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTg > 1:334794/1‑139 (MQ=255) ggggCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGa < 1:736306/51‑1 (MQ=255) ggggCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGa > 2:736306/1‑51 (MQ=255) ggggCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGtt < 1:557822/57‑1 (MQ=255) ggggCAACGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGtt > 2:557822/1‑57 (MQ=255) cGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACg > 2:206886/1‑139 (MQ=255) cGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACg > 2:265677/1‑139 (MQ=255) cGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACg > 1:447551/1‑139 (MQ=255) cGTACCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACg > 1:118382/1‑139 (MQ=255) | GCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACG > NC_000913/2833943‑2834212 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |