Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,066,288	T→C	T296A (ACG→GCG)	ygfI ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,066,288	0	T	C	100.0%	144.3 / NA	39	T296A (ACG→GCG)	ygfI	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/19);  total (20/19)

TCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCAT  >  NC_000913/3066163‑3066392                                                                                                                              |                                                                                                         tCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCt                                                                                             >  1:181786/1‑139 (MQ=255)   gggATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTc                                                                                           >  1:149557/1‑139 (MQ=255)     gATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCa                                                                                                   >  1:152180/1‑129 (MQ=255)     gATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCa                                                                                                   <  2:152180/129‑1 (MQ=255)     gATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAg                                                                                         <  2:159198/139‑1 (MQ=255)       ttATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCa                                                                                       >  1:16967/1‑139 (MQ=255)         aTCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAat                                                                                     >  2:191431/1‑139 (MQ=255)         aTCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAat                                                                                     >  1:564289/1‑139 (MQ=255)          tCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAata                                                                                    >  2:614085/1‑139 (MQ=255)                    tATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCa                                                                          >  2:728974/1‑139 (MQ=255)                           aaaaTGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGc                                                                   >  2:287235/1‑139 (MQ=255)                              aTGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGt                                                                <  1:359133/139‑1 (MQ=255)                                gcgcCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGtat                                                              >  1:721245/1‑139 (MQ=255)                                     tGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACga                                                         <  2:53478/139‑1 (MQ=255)                                                ttCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGAt                                              <  1:724923/139‑1 (MQ=255)                                                     cacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAt                                         <  2:716922/139‑1 (MQ=255)                                                     cacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAt                                         <  2:378368/139‑1 (MQ=255)                                                     cacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAt                                         >  1:14471/1‑139 (MQ=255)                                                     cacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAt                                         <  1:191431/139‑1 (MQ=255)                                                         aTCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAg                                     >  1:537951/1‑139 (MQ=255)                                                          tCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGa                                    <  2:14471/139‑1 (MQ=255)                                                            ttAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGata                                  <  2:564289/139‑1 (MQ=255)                                                            ttAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGata                                  <  2:721245/139‑1 (MQ=255)                                                              aGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATAtt                                <  1:287235/139‑1 (MQ=255)                                                               gCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTc                               >  1:90668/1‑139 (MQ=255)                                                                    gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCa                          >  2:278370/1‑139 (MQ=255)                                                                    gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCa                          >  1:292200/1‑139 (MQ=255)                                                                               acacCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGc               <  1:82399/139‑1 (MQ=255)                                                                                cacCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                    >  2:356181/1‑85 (MQ=255)                                                                                cacCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                    <  1:356181/85‑1 (MQ=255)                                                                                cacCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGata                                  >  2:213041/1‑119 (MQ=255)                                                                                cacCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGcc              >  1:419103/1‑139 (MQ=255)                                                                                cacCGTAAATTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGata                                  <  1:213041/119‑1 (MQ=255)                                                                                   cGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCa                                                                                                   >  2:197722/1‑51 (MQ=255)                                                                                   cGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCa                                                                                                   <  1:197722/51‑1 (MQ=255)                                                                                            attGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCAt  <  2:645347/139‑1 (MQ=255)                                                                                            attGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCAt  <  2:90668/139‑1 (MQ=255)                                                                                             ttGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                    <  2:294603/72‑1 (MQ=255)                                                                                             ttGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                    >  1:294603/1‑72 (MQ=255)                                                                                                                              |                                                                                                         TCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCAT  >  NC_000913/3066163‑3066392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑