Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,160,077 T→C Y357C (TAC→TGC)  ygiQ ← Radical SAM superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,160,0770TC100.0% 146.8 / NA 40Y357C (TAC→TGC) ygiQRadical SAM superfamily protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/21);  total (19/21)

TGCGCCCTTCGTGCTCGGTGATAGAACAGAAAGAGCAGCCGCCAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGTATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTG  >  NC_000913/3159943‑3160204
                                                                                                                                      |                                                                                                                               
tGCGCCCTTCGTGCTCGGTGATAGAACAGAAAGAGCAGCCGCCAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATgg                                                                                                                             <  2:448756/139‑1 (MQ=255)
tGCGCCCTTCGTGCTCGGTGATAGAACAGAAAGAGCAGCCGCCAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATgg                                                                                                                             >  2:527645/1‑139 (MQ=255)
               cGGTGATAGAACAGAAAGAGCAGCCGCCAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCt                                                                                                              <  2:64378/139‑1 (MQ=255)
                              aaGAGCAGCCGCCAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGt                                                                                               >  2:84944/1‑139 (MQ=255)
                               agagCAGCCGCCAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTa                                                                                              <  2:587902/139‑1 (MQ=255)
                                 agcagcCGCCAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACg                                                                                                                >  2:798444/1‑119 (MQ=255)
                                 agcagcCGCCAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACg                                                                                                                <  1:798444/119‑1 (MQ=255)
                                  gcagcCGCCAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGaa                                                                                           >  2:279959/1‑139 (MQ=255)
                                  gcagcCGCCAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGaa                                                                                           >  2:575255/1‑139 (MQ=255)
                                   cagcCGCCAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGaaa                                                                                          >  1:114131/1‑139 (MQ=255)
                                   cagcCGCCAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCCGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGaaa                                                                                          >  1:474323/1‑139 (MQ=255)
                                     gccgccAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGc                                                                                        >  1:55069/1‑139 (MQ=255)
                                        gccAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACg                                                                                                                >  2:550299/1‑112 (MQ=255)
                                        gccAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACg                                                                                                                <  1:550299/112‑1 (MQ=255)
                                                  ccACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCg                                                                           <  1:294988/139‑1 (MQ=255)
                                                    aCGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCggg                                                                         <  2:143453/139‑1 (MQ=255)
                                                    aCGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCggg                                                                         <  2:346379/139‑1 (MQ=255)
                                                      gCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAATCGGAATAGCAGGCGGGtt                                                                       <  1:445860/139‑1 (MQ=255)
                                                      gCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGtt                                                                       <  2:474323/139‑1 (MQ=255)
                                                      gCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGtt                                                                       <  2:114131/139‑1 (MQ=255)
                                                      gCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGtt                                                                       <  1:717773/139‑1 (MQ=255)
                                                        aTAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGa                                                                     <  2:55069/139‑1 (MQ=255)
                                                                gACCGAAAAACGGATCATTTCGTAAGCAGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCcacaca                                                             >  2:646063/1‑139 (MQ=255)
                                                                                          ccGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCaa                                   <  2:793240/139‑1 (MQ=255)
                                                                                              aaTACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATgcg                               <  2:780386/139‑1 (MQ=255)
                                                                                                   gggCATTGCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGc                          <  1:646063/139‑1 (MQ=255)
                                                                                                          gCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGtt                                                                       >  2:456462/1‑87 (MQ=255)
                                                                                                          gCCATAGGCCGGATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGtt                                                                       <  1:456462/87‑1 (MQ=255)
                                                                                                                     gATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCggg                                                                         <  2:744488/74‑1 (MQ=255)
                                                                                                                     gATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCggg                                                                         >  1:744488/1‑74 (MQ=255)
                                                                                                                     gATGTGGCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTc        >  1:209664/1‑139 (MQ=255)
                                                                                                                          ggCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtggt   >  2:386953/1‑139 (MQ=255)
                                                                                                                           gCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtggtg  >  2:796978/1‑139 (MQ=255)
                                                                                                                           gCACGCGCTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtggtg  >  1:212904/1‑139 (MQ=255)
                                                                                                                               gcgcTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtggtg  >  2:465482/1‑135 (MQ=255)
                                                                                                                               gcgcTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTAGTTTCgtgttg  <  1:465482/135‑1 (MQ=255)
                                                                                                                                cgcTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCgt                                                >  1:93432/1‑88 (MQ=255)
                                                                                                                                cgcTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCgt                                                <  2:93432/88‑1 (MQ=255)
                                                                                                                                cgcTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATgcg                               <  2:482670/105‑1 (MQ=255)
                                                                                                                                cgcTTGCATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATgcg                               >  1:482670/1‑105 (MQ=255)
                                                                                                                                      |                                                                                                                               
TGCGCCCTTCGTGCTCGGTGATAGAACAGAAAGAGCAGCCGCCAAAGCAGCCACGCATAATGTTGACCGAAAAACGGATCATTTCGTAAGCCGGAATACGGGCATTGCCATAGGCCGGATGTGGCACGCGCTTGTATGGCAGCGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTG  >  NC_000913/3159943‑3160204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑