Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,790,538 A→G S270P (TCT→CCT)  tdh ← L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,790,5380AG100.0% 159.8 / NA 42S270P (TCT→CCT) tdhL‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/20);  total (22/20)

ATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACT  >  NC_000913/3790404‑3790669
                                                                                                                                      |                                                                                                                                   
aTGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCata                                                                                                                                 >  2:531723/1‑139 (MQ=255)
     tAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGa                                                                                                                            <  2:428113/139‑1 (MQ=255)
         gATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGAcggc                                                                                                                        >  2:81723/1‑139 (MQ=255)
            cGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGa                                                                                                                     <  1:531723/139‑1 (MQ=255)
             ggCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGaa                                                                                                                    >  1:461285/1‑139 (MQ=255)
              gCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAAt                                                                                                                   >  2:483095/1‑139 (MQ=255)
               cGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATa                                                                                                                  >  1:255962/1‑139 (MQ=255)
                 aaaGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATAcc                                                                                                                <  2:375141/139‑1 (MQ=255)
                    gATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAg                                                                                                             <  2:392506/139‑1 (MQ=255)
                       cGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCAt                                                                                                          <  2:102768/139‑1 (MQ=255)
                                  tGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGgc                                                                                               <  2:461285/139‑1 (MQ=255)
                                            ccgccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGAtt                                                                                     >  1:546035/1‑139 (MQ=255)
                                              gccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCa                                                                                   >  2:296913/1‑139 (MQ=255)
                                                        aCCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAg                                                                         <  2:279922/139‑1 (MQ=255)
                                                            ggTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATg                                                                     >  2:195129/1‑139 (MQ=255)
                                                                        ctcACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGc                                                                        <  1:331769/124‑1 (MQ=255)
                                                                        ctcACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGc                                                                        >  2:331769/1‑124 (MQ=255)
                                                                                  tAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCa                                                                                   >  1:783370/1‑103 (MQ=255)
                                                                                  tAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCa                                                                                   <  2:783370/103‑1 (MQ=255)
                                                                                  tAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATg                                                                                 >  2:163306/1‑105 (MQ=255)
                                                                                  tAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATg                                                                                 <  1:163306/105‑1 (MQ=255)
                                                                                      tACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGa                                           <  1:81723/139‑1 (MQ=255)
                                                                                      tACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGa                                           <  1:483095/139‑1 (MQ=255)
                                                                                         cTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACAt                                        >  2:352382/1‑139 (MQ=255)
                                                                                            tAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTc                                     >  2:279703/1‑139 (MQ=255)
                                                                                             aaTGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGgc                                                                                               <  1:500542/80‑1 (MQ=255)
                                                                                             aaTGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGgc                                                                                               >  2:500542/1‑80 (MQ=255)
                                                                                             aaTGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGa                                           >  2:703000/1‑132 (MQ=255)
                                                                                             aaTGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGa                                           <  1:703000/132‑1 (MQ=255)
                                                                                                      gCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGa                                                               >  1:65795/1‑103 (MQ=255)
                                                                                                      gCCTTTAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGa                                                               <  2:65795/103‑1 (MQ=255)
                                                                                                           tAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGAcc                               <  1:568580/130‑1 (MQ=255)
                                                                                                           tAAAGATCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGAcc                               >  2:568580/1‑130 (MQ=255)
                                                                                                                aTCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCtt                 >  2:360435/1‑139 (MQ=255)
                                                                                                                aTCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCtt                 >  1:370345/1‑139 (MQ=255)
                                                                                                                aTCACTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCtt                 >  2:302349/1‑139 (MQ=255)
                                                                                                                   aCTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCgg              <  1:306127/139‑1 (MQ=255)
                                                                                                                   aCTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCgg              <  1:302349/139‑1 (MQ=255)
                                                                                                                    cTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGt             <  1:785090/139‑1 (MQ=255)
                                                                                                                    cTTTGGTCCAGTCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGt             <  1:195129/139‑1 (MQ=255)
                                                                                                                               tCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGATATCAAAACCTTCGGTCATGCCTAACt  >  1:68797/1‑139 (MQ=255)
                                                                                                                               tCGATAGGCATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACt  >  2:422323/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                   
ATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACT  >  NC_000913/3790404‑3790669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑