Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,959,758 A→G L19P (CTG→CCG)  ppiC ← peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,7580AG100.0% 113.5 / NA 31L19P (CTG→CCG) ppiCpeptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/19);  total (12/19)

TTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAG  >  NC_000913/3959623‑3959893
                                                                                                                                       |                                                                                                                                       
ttATCGAACGCGGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCggag                                                                                                                                      <  2:520571/139‑1 (MQ=255)
        cGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTtc                                                                                                                              <  1:56834/139‑1 (MQ=255)
         gCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTtct                                                                                                                             <  1:6720/139‑1 (MQ=255)
                  aTCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTa                                                                                                                    <  1:486271/139‑1 (MQ=255)
                   tCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTAc                                                                                                                   >  1:14261/1‑139 (MQ=255)
                                       ccTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGtgctgctg                                                                                               <  2:373490/139‑1 (MQ=255)
                                          aaaTCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGctgctgctg                                                                                            <  1:431392/139‑1 (MQ=255)
                                                ccgccgCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCtcttc                                                                                                                         >  1:292900/1‑104 (MQ=255)
                                                ccgccgCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCtcttc                                                                                                                         <  2:292900/104‑1 (MQ=255)
                                                   ccgcGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCa                                                                                   <  1:64789/139‑1 (MQ=255)
                                                             cTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGcc                                                                         >  2:271335/1‑139 (MQ=255)
                                                                 tGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGa                                                                     <  1:108176/139‑1 (MQ=255)
                                                                  gggCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAg                                                                    >  1:50834/1‑139 (MQ=255)
                                                                   ggCAAATGGAGTGTTTCTTCGCCAGCTTGTCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGt                                                                   <  2:542006/139‑1 (MQ=255)
                                                                   ggCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGt                                                                   <  1:746232/139‑1 (MQ=255)
                                                                    gCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTg                                                                  <  2:175646/139‑1 (MQ=255)
                                                                      aaaTGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGaa                                                                <  2:50834/139‑1 (MQ=255)
                                                                             gtgtTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGt                                                         >  2:781493/1‑139 (MQ=255)
                                                                              tgtTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAgg                                                                                                               >  2:669005/1‑84 (MQ=255)
                                                                              tgtTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAgg                                                                                                               <  1:669005/84‑1 (MQ=255)
                                                                                    ttcGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAggcgg                                                  <  1:781493/139‑1 (MQ=255)
                                                                                    ttcGCCAGCTTGCCGAAATCGGCCCCGATCGTCATTTGCTCGAAAAGGCACGGAGCCTGATACTCTTCTTTTAAAACGTGATGCAGTGCGCCTGCTGTTTTTAACATGATCGTGCCATGAGTGAACAATTGTCaaacgg                                                  <  1:61235/139‑6 (MQ=255)
                                                                                            cTTGCTGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGtgctgctg                                                                                               <  2:576951/86‑1 (MQ=255)
                                                                                            cTTGCTGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGtgctgctg                                                                                               >  1:576951/1‑86 (MQ=255)
                                                                                                               ttCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGc                                        >  1:497158/1‑122 (MQ=255)
                                                                                                               ttCTTAATCTGCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGc                                        <  2:497158/122‑1 (MQ=255)
                                                                                                                         gCTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTgt             <  2:14261/139‑1 (MQ=255)
                                                                                                                          cTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTgtc            >  1:474692/1‑139 (MQ=255)
                                                                                                                          cTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTgtc            >  1:774835/1‑139 (MQ=255)
                                                                                                                          cTCGAGAAGATCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATCGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTgtc            >  1:657518/1‑139 (MQ=255)
                                                                                                                                   aTCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGa   <  1:271335/139‑1 (MQ=255)
                                                                                                                                    tCCGGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAg  >  2:465593/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAG  >  NC_000913/3959623‑3959893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑