| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 467,458 | C→T | P16L (CCC→CTC) | cof → | thiamine pyrimidine pyrophosphate hydrolase, HMP‑PP phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 467,458 | 0 | C | T | 94.8% | 179.9 / 1.5 | 58 | P16L (CCC→CTC) | cof | thiamine pyrimidine pyrophosphate hydrolase, HMP‑PP phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (33/22); total (35/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
AATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGT > NC_000913/467326‑467592 | aaTATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACca < 1:284986/139‑1 (MQ=255) tttAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTa > 1:628700/1‑139 (MQ=255) tttAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTa < 1:279272/139‑1 (MQ=255) tttAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTa > 2:585888/1‑139 (MQ=255) tcaCTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATACGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCctc > 1:481691/3‑139 (MQ=255) cTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCctctct > 2:280104/1‑139 (MQ=255) ccGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACt > 1:123977/1‑139 (MQ=255) ccGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTCATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACt > 1:485297/1‑139 (MQ=255) gTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGaaa < 1:385469/125‑1 (MQ=255) gTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGaaa > 2:385469/1‑125 (MQ=255) tCAGGTGATACTCAATCACCATTAACCGAGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGa < 2:481691/139‑1 (MQ=255) cAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACcatca < 1:398503/104‑1 (MQ=255) cAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACcatca > 2:398503/1‑104 (MQ=255) gTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGc > 2:693526/1‑139 (MQ=255) gATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAAcc > 1:49153/1‑115 (MQ=255) gATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAAcc < 2:49153/115‑1 (MQ=255) tACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGCTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGa > 2:502862/1‑139 (MQ=255) cTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAAc > 1:62893/1‑139 (MQ=255) cTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAAc > 1:138307/1‑139 (MQ=255) tCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAAcg < 2:485297/139‑1 (MQ=255) tCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAAcg < 2:123977/139‑1 (MQ=255) tCAATCACCATTAACCGTGTCACAGAGTCGAGACAGAAATGGCTCGTCTGGCAGCATTTCAAATGGATGGCCCTTTATTGCCGCTCGACCATCATTTAAGTGCGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAAcg < 1:481826/139‑1 (MQ=255) tCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGAc > 2:431486/1‑139 (MQ=255) tgtCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACtt > 1:275282/1‑139 (MQ=255) gtCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACttt > 1:343212/1‑139 (MQ=255) gtCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACttt > 1:641325/1‑139 (MQ=255) gtCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACttt > 2:629239/1‑139 (MQ=255) gtCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACttt > 2:217678/1‑139 (MQ=255) gtCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACAt < 1:656571/127‑1 (MQ=255) gtCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACAt > 2:656571/1‑127 (MQ=255) tGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACggg > 2:87712/1‑139 (MQ=255) tGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACggg < 2:628700/139‑1 (MQ=255) tGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACggg > 1:516420/1‑139 (MQ=255) tGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACggg < 1:585888/139‑1 (MQ=255) gagaAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGc > 2:165065/1‑139 (MQ=255) gaaaTGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATg > 2:182056/1‑139 (MQ=255) aaTGGCTCGTCTGGCAGCATTTGATATTGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATgcg > 1:581712/1‑139 (MQ=255) tGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCt > 2:295068/1‑139 (MQ=255) cTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCctct < 1:126335/72‑1 (MQ=255) cTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCctct > 2:126335/1‑72 (MQ=255) tCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATg > 2:461088/1‑139 (MQ=255) tCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATg < 1:280104/139‑1 (MQ=255) tCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATg < 2:138307/139‑1 (MQ=255) ggCAGCATTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATgcag < 1:629239/139‑1 (MQ=255) aTTTGATATGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATAtt < 2:275282/139‑1 (MQ=255) tttGATAGGGATGGCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATtc > 1:463491/1‑139 (MQ=255) ggatggCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGgcg < 1:461088/139‑1 (MQ=255) ggatggCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGgcg < 1:115880/139‑1 (MQ=255) atggCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCt > 1:788250/1‑139 (MQ=255) ggCACTTTATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTAt < 1:87712/139‑1 (MQ=255) tATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTAt < 2:446906/132‑1 (MQ=255) tATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTAt > 1:446906/1‑132 (MQ=255) tATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGa > 2:472038/1‑139 (MQ=255) tATTGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGa > 2:182828/1‑139 (MQ=255) ttGATGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATg < 1:502862/139‑1 (MQ=255) aTGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGAGATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATg > 1:524172/1‑105 (MQ=255) aTGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATg < 2:524172/105‑1 (MQ=255) aTGCTCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGt > 2:321642/1‑139 (MQ=255) | AATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGTGTCACAGAGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGT > NC_000913/467326‑467592 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |