Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,024,1390TC42.5% 12.0 / 57.7 47F58L (TTC→CTC) rcsAtranscriptional regulator of colanic acid capsular biosynthesis
Reads supporting (aligned to +/- strand):  ref base T (14/13);  new base C (9/11);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 7.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GGTTTAACCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTAT  >  NC_000913/2024010‑2024271
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ggTTTAACCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTCTCATCCACg                                                                                                                             <  2:23417/139‑1 (MQ=255)
ggTTTAACCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTCTCATCCACg                                                                                                                             <  2:447136/139‑1 (MQ=255)
          ggTATCTGTTGAGTAGAGAGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTCTCATCCACGATGCTACTaa                                                                                                                   >  2:461252/1‑139 (MQ=255)
           gTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTCTCATCCACGATGCTTCTAAc                                                                                                                  >  1:224704/1‑139 (MQ=255)
              tCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTCTCATCCACGATGCTTCTAACAGt                                                                                                               <  1:284742/139‑1 (MQ=255)
                       tAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTCTCATCCACGATGCTTCTAACAGTCAGCGTATc                                                                                                      <  2:630866/139‑1 (MQ=255)
                          atgggCTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAg                                                                                                   >  1:450144/3‑139 (MQ=255)
                          agGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAg                                                                                                   >  1:87852/1‑139 (MQ=255)
                               ttAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCtcat                                                                                              >  1:695693/1‑139 (MQ=255)
                                        gagaAATCAACGACATTGAAACCGTTGATGACCTTGCCATACCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCa                                                                                     >  1:430382/1‑139 (MQ=255)
                                         agaAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCaa                                                                                    <  2:430382/139‑1 (MQ=255)
                                         agaAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTCTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCaa                                                                                    <  2:224704/139‑1 (MQ=255)
                                          gaAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTCTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAAc                                                                                   <  2:446639/139‑1 (MQ=255)
                                                aaCGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCa                                                                             <  2:695693/139‑1 (MQ=255)
                                                  cGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAAt                                                                           <  2:87852/139‑1 (MQ=255)
                                                  cGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCGTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAAt                                                                           <  2:450144/139‑1 (MQ=255)
                                                     cATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCa                                                                                                                               <  2:629706/84‑1 (MQ=255)
                                                     cATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCa                                                                                                                               >  1:629706/1‑84 (MQ=255)
                                                        tGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTCTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGATCATCATTAATCAACATCTCAATACGtta                                                                     >  2:394076/1‑139 (MQ=255)
                                                                ttGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTa                                                                                                                    <  1:6578/84‑1 (MQ=255)
                                                                ttGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTa                                                                                                                    >  2:6578/1‑84 (MQ=255)
                                                                ttGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTCTCATCCACg                                                                                                                             >  1:94858/1‑75 (MQ=255)
                                                                ttGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTCTCATCCACg                                                                                                                             <  2:94858/75‑1 (MQ=255)
                                                                                    tgtgATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACg                                                                                                                             <  1:91673/55‑1 (MQ=255)
                                                                                    tgtgATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACg                                                                                                                             >  2:91673/1‑55 (MQ=255)
                                                                                      tgATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACg                                                                                                                             >  1:94052/1‑53 (MQ=255)
                                                                                      tgATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACg                                                                                                                             <  2:94052/53‑1 (MQ=255)
                                                                                      tgATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAAt                                                 >  1:667827/1‑129 (MQ=255)
                                                                                      tgATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAAt                                                 <  2:667827/129‑1 (MQ=255)
                                                                                          tCACAGCGCCCTTTAGTGGTGTTAATTAATGAGGACTGTCTCATCCACGATGCTGCTAACAGTCAGCGTATCAAGCTCAGCATTCATCAACATCCCAATGCGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCAtt                                   <  1:461252/139‑1 (MQ=255)
                                                                                           cacaGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACg                                                                                                                             <  1:108155/48‑1 (MQ=255)
                                                                                           cacaGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACg                                                                                                                             >  2:108155/1‑48 (MQ=255)
                                                                                           cacaGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTCTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTTCCAAt                                          >  1:698460/1‑131 (MQ=255)
                                                                                           cacaGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTCTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAAt                                          <  2:698460/131‑1 (MQ=255)
                                                                                                               tttATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCAtt                                   >  2:105052/1‑118 (MQ=255)
                                                                                                               tttATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCAtt                                   <  1:105052/118‑1 (MQ=255)
                                                                                                               tttATTAATGAGGACTGTCTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCa              >  1:46200/1‑139 (MQ=255)
                                                                                                                    taATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAat                          <  2:613549/122‑1 (MQ=255)
                                                                                                                    taATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAat                          >  1:613549/1‑122 (MQ=255)
                                                                                                                    taATGAGGACTGTCTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCt                                                                                                 <  2:622343/51‑1 (MQ=255)
                                                                                                                    taATGAGGACTGTCTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCt                                                                                                 >  1:622343/1‑51 (MQ=255)
                                                                                                                         aGGACTGTCTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAAttt    >  1:531978/1‑139 (MQ=255)
                                                                                                                           gACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTtgatga                            <  2:160475/113‑1 (MQ=255)
                                                                                                                           gACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTtgatga                            >  1:160475/1‑113 (MQ=255)
                                                                                                                           gACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTAt  >  1:460409/1‑139 (MQ=255)
                                                                                                                           gACTGTCTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTa   <  1:95224/138‑1 (MQ=255)
                                                                                                                           gACTGTCTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTa   >  2:95224/1‑138 (MQ=255)
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GGTTTAACCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGATGCTTCTAACAGTCAGCGTATCAAGCTCATCATTAATCAACATCCCAATACGTTATTTATCGTTTTTATGGCAATTGCCAATGTTCATTTTGATGAATATCTATTGGTCAGAAAAAATTTAT  >  NC_000913/2024010‑2024271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑