Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,393,315	T→C	V30A (GTA→GCA)	mppA →	murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,393,315	0	T	C	100.0%	139.5 / NA	37	V30A (GTA→GCA)	mppA	murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/17);  total (20/17)

TCAATTTTAATGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGTACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAATCAGAACGAA  >  NC_000913/1393197‑1393448                                                                                                                       |                                                                                                                                      tCAATTTTAATGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGc                                                                                                                   >  1:687208/1‑139 (MQ=255)         aaTGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTgcgc                                                                                                           <  2:687208/139‑1 (MQ=255)         aaTGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTgcgc                                                                                                           <  1:386366/139‑1 (MQ=255)               ggTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACAtt                                                                                                     >  2:632145/1‑139 (MQ=255)               ggTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACAtt                                                                                                     >  2:232852/1‑139 (MQ=255)                          aaCTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGcc                                                                                          >  2:693017/1‑139 (MQ=255)                            cTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTg                                                                                        <  1:632145/139‑1 (MQ=255)                            cTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTg                                                                                        >  1:565750/1‑139 (MQ=255)                               aTGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTgcgc                                                                                                           <  1:351623/117‑1 (MQ=255)                               aTGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTgcgc                                                                                                           >  2:351623/1‑117 (MQ=255)                                       ctctGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCcaca                                                                                                       >  1:357411/1‑113 (MQ=255)                                       ctctGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCcaca                                                                                                       <  2:357411/113‑1 (MQ=255)                                       ctctGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGAt                                                                             >  2:613409/1‑139 (MQ=255)                                             ttCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCt                                                                       >  2:203607/1‑139 (MQ=255)                                                  tCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGc                                                                  >  2:449426/1‑139 (MQ=255)                                                    aCGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCg                                                                >  2:317177/1‑139 (MQ=255)                                                                cTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCag                                                    >  1:357062/1‑139 (MQ=255)                                                                   ttGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAgaga                                                 >  1:91668/1‑139 (MQ=255)                                                                           cagcaTTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCt                                                                                 <  1:59497/99‑1 (MQ=255)                                                                           cagcaTTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCt                                                                                 >  2:59497/1‑99 (MQ=255)                                                                           cagcaTTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTc                                         >  2:304695/1‑139 (MQ=255)                                                                           cagcaTTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTc                                         >  1:339076/1‑139 (MQ=255)                                                                              caTTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCAtt                                      <  1:304695/139‑1 (MQ=255)                                                                                  tctcTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTcgcg                                  <  1:118556/139‑1 (MQ=255)                                                                                  tctcTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTcgcg                                  <  1:693017/139‑1 (MQ=255)                                                                                    tctTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGAt                                <  2:247142/139‑1 (MQ=255)                                                                                    tctTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGAt                                <  2:46462/139‑1 (MQ=255)                                                                                                tgcAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTg                                                                                        >  1:688828/1‑71 (MQ=255)                                                                                                tgcAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTg                                                                                        <  2:688828/71‑1 (MQ=255)                                                                                                 gcAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTc                   <  1:232852/139‑1 (MQ=255)                                                                                                  cAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACAtt                                                                                                     >  1:195112/1‑56 (MQ=255)                                                                                                  cAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACAtt                                                                                                     <  2:195112/56‑1 (MQ=255)                                                                                                  cAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGc                                                           <  2:473768/98‑1 (MQ=255)                                                                                                  cAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGc                                                           >  1:473768/1‑98 (MQ=255)                                                                                                          ccGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGt                                          <  1:14646/107‑1 (MQ=255)                                                                                                          ccGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGt                                          >  2:14646/1‑107 (MQ=255)                                                                                                                  cacaGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAATCAGAACGaa  <  1:317177/139‑1 (MQ=255)                                                                                                                       |                                                                                                                                      TCAATTTTAATGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGTACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTGGTGAATCAGAACGAA  >  NC_000913/1393197‑1393448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑