Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,670,695	A→G	M333V (ATG→GTG)	ynfM →	putative arabinose efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,670,695	0	A	G	100.0%	72.1 / NA	20	M333V (ATG→GTG)	ynfM	putative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/11);  total (9/11)

AGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTAGCCAGCAGCTGGATCGGCCCCCGCGCAAAACGCGCTAAAGGCCAGGCCTCCTCGCTGTATCTGTTCAGTTACT  >  NC_000913/1670566‑1670809                                                                                                                                  |                                                                                                                   aGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTActct                                                                                                           >  1:253892/1‑139 (MQ=255)                     cATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCa                                                                                       <  1:294450/139‑1 (MQ=255)                        gACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGcc                                                                                    <  1:306493/139‑1 (MQ=255)                                    tGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTAGcc                                                                        <  2:253892/139‑1 (MQ=255)                                        cGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTAGCcagca                                                                    >  1:113184/1‑139 (MQ=255)                                                       ttgttTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGAttcttc                                                                                          <  1:545817/102‑1 (MQ=255)                                                       ttgttTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGAttcttc                                                                                          >  2:545817/1‑102 (MQ=255)                                                       ttgttTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTa                                                                           >  2:203531/1‑117 (MQ=255)                                                       ttgttTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTa                                                                           <  1:203531/117‑1 (MQ=255)                                                                               tttGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTAGCCAGCAGCTGGATCGGCCCCCGCGCAAAACGCGCTAAaggccagg                             <  2:113184/139‑1 (MQ=255)                                                                               tttGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTAGCCAGCAGCTGGATCGGCCCCCGCGCAAAACGCGCTAAaggccagg                             >  2:629016/1‑139 (MQ=255)                                                                                   gTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTAGCCAGCAGCTGGATCGGCCCCCGCGCAAAACGCGCTAAAGGCCAGGcctc                         <  2:269566/139‑1 (MQ=255)                                                                                            tgaCCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTAGCCAGCAGCTGGATCGGCCCCCGCGCAAAACGCGCTAAAGGCCAGGCCTCCTCGCTGTa                >  1:375388/1‑139 (MQ=255)                                                                                             gaCCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTAGCCAGCAGCTGGATCGGCCCCCGCGCAAAACGCGCTAAAGGCCAGGCCTCCTCGCTGTAt               >  2:497066/1‑139 (MQ=255)                                                                                              aCCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTAGCCAGCAGCTGGATCGGCCCCCGCGCAAAACGCGCTAAAGGCCAGGCCTCCTCGCTGTATc              <  1:629016/139‑1 (MQ=255)                                                                                                  tATTCAGCTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTAGCCAGCAGCTGGATCGGCCCCCGCGCAAAACGCGCTAAAGGCCAGGCCTCCTCGCTGTATCTGtt          <  2:375388/139‑1 (MQ=255)                                                                                                      cAGCTCGCTGTTGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTAGCCAGCAGCTGGATCGGCCCCCGCGCAAAACGCGCTAAAGGCCAGGCCTCCTCGCTGTATCTGTTCAGt      <  1:123140/139‑1 (MQ=255)                                                                                                         cTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTAGCCAGCAGCTGGATCGGCCCCCGCGCAAAACGCGCTAAAGGCCAGGCCTCCTCGCTGTATc              >  2:374900/1‑128 (MQ=255)                                                                                                         cTCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTAGCCAGCAGCTGGATCGGCCCCCGCGCAAAACGCGCTAAAGGCCAGGCCTCCTCGCTGTATc              <  1:374900/128‑1 (MQ=255)                                                                                                          tCGCTGTGGCTGATCTTTGCCGGAGTGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTAGCCAGCAGCTGGATCGGCCCCCGCGCAAAACGCGCTAAAGGCCAGGCCTCCTCGCTGTATCTGTTCAGTTACt  >  2:69261/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                   AGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCAGGATTCTTCGCAGCCCACTCAGTAGCCAGCAGCTGGATCGGCCCCCGCGCAAAACGCGCTAAAGGCCAGGCCTCCTCGCTGTATCTGTTCAGTTACT  >  NC_000913/1670566‑1670809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑