Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,715,061	T→C	K277E (AAA→GAA)	pdxY ←	pyridoxamine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,715,061	0	T	C	100.0%	61.2 / NA	17	K277E (AAA→GAA)	pdxY	pyridoxamine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/10);  total (7/10)

TATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGAGCAGCCACCACTTGCAGCTCATATTCCTGCATTGCTTTGGTGGTCACCATGATTTCGTAGACTGCAGCGGTCACATGTTCCAGCGCCTCCTGCAGCGTTG  >  NC_000913/1714928‑1715177                                                                                                                                      |                                                                                                                     tATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTCGGCaa                                                                                                                 >  2:477913/1‑139 (MQ=255)         aaCGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTCGGCAATa                                                                                                               <  1:493661/133‑1 (MQ=255)         aaCGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTCGGCAATa                                                                                                               >  2:493661/1‑133 (MQ=255)              ccGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTCGGCAATACGATCCTGagc                                                                                                    <  1:393825/139‑1 (MQ=255)                              cgcTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTCGGCAATACGATCCTGAGCAGCCACCACTTGCAGc                                                                                    <  1:278085/139‑1 (MQ=255)                                             gCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTCGGCAATACGATCCTGAGCAGccacc                                                                                             <  2:538798/115‑1 (MQ=255)                                             gCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTCGGCAATACGATCCTGAGCAGccacc                                                                                             >  1:538798/1‑115 (MQ=255)                                                                               gtgGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTCGGCAATACGATCCTGAGCAGCCACCACTTGCAGCTCATATTCCTGCATTGCTTTGGTGGTCACCATGATTTCGTAGACTGCAg                                   <  1:477913/139‑1 (MQ=255)                                                                                tgGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTCGGCAATACGATCCTGAGCAGCCACCACTTGCAGCTCATATTCCTGCATTGCTTTGGTGGTCACCATGATTTCGTAGACTGCAGc                                  >  1:111750/1‑139 (MQ=255)                                                                                 gggCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTCGGCAATACGATCCTGAGCAGCCACCACTTGCAGCTCATATTCCTGCATTGCTTTGGTGGTCACCATGATTTCGTAGACTGCAGCg                                 >  1:550961/1‑139 (MQ=255)                                                                                  ggCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTCGGCAATACGATCCTGAGCAGCCACCACTTGCAGCTCATATTCCTGCATTGCTTTGGTGGTCACCATGATTTCGTAGACTGCAGCgg                                <  2:550961/139‑1 (MQ=255)                                                                                                atTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTCGGCAATACGATCCTGAGCAGCCACCACTTGCAGCTCATATTCCTGCATTGCTTTGTTGGTCACCATGATTTCGTAGACTGCAGCGGTCACATGTTCCAgc                  <  1:494847/139‑1 (MQ=255)                                                                                                            tgttgCGCTGAAGTAATGTTCTGGTTCGGCAATACGATCCTGAGCAGCCACCACTTGCAGCTCATATTCCTGCATTGCTTTGGTGGTCACCATGATTTCGTAGACTGCAGCGGTcaca                           >  2:441959/1‑118 (MQ=255)                                                                                                            tgttgCGCTGAAGTAATGTTCTGGTTCGGCAATACGATCCGGAGCAGCCACCACTTGCAGCTCATATTCCTGCATTGCTTTGGTGGTCACCATGATTTCGTAGACTGCAGCGGTcaca                           <  1:441959/118‑1 (MQ=255)                                                                                                                gcgcTGAAGTAATGTTCTGGTTCGGCAATACGATCCTGAGCAGCCACCACTTGCAGCTCATATTCCTGCATTGCTTTGGTGGTCACCATGATTTCGTAGACTGCAGCGGTCACATGTTCCAGCGCCTCCTGCAGCGTTg  <  2:111750/139‑1 (MQ=255)                                                                                                                 cgcTGAAGTAATGTTCTGGTTCGGCAATACGATCCTGAGCAGCCACCACTTGCAGCTCATATTCCTGCATTGCTTTGGTGGTCACCATGATTTCGTAGACTGCAGCGGTcaca                           <  1:89864/113‑1 (MQ=255)                                                                                                                 cgcTGAAGTAATGTTCTGGTTCGGCAATACGATCCTGAGCAGCCACCACTTGCAGCTCATATTCCTGCATTGCTTTGGTGGTCACCATGATTTCGTAGACTGCAGCGGTcaca                           >  2:89864/1‑113 (MQ=255)                                                                                                                                      |                                                                                                                     TATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGAGCAGCCACCACTTGCAGCTCATATTCCTGCATTGCTTTGGTGGTCACCATGATTTCGTAGACTGCAGCGGTCACATGTTCCAGCGCCTCCTGCAGCGTTG  >  NC_000913/1714928‑1715177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑