Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,374	G→A	G226D (GGC→GAC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,374	0	G	A	100.0%	109.3 / NA	33	G226D (GGC→GAC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/16);  total (17/16)

GTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTAT  >  NC_000913/1756239‑1756500                                                                                                                                        |                                                                                                                               gTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCt                                                                                                                             >  2:370003/1‑139 (MQ=255)     cGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTcaac                                                                                                                         >  2:677933/1‑139 (MQ=255)                      ttgttgCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCt                                                                                                        >  1:371221/1‑139 (MQ=255)                                            tAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATg                                                                                  >  2:393934/1‑139 (MQ=255)                                             aaGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATgg                                                                                 >  2:44609/1‑139 (MQ=255)                                              aGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGt                                                                                >  2:5709/1‑139 (MQ=255)                                                  ttCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTgcg                                                                            >  2:102885/1‑139 (MQ=255)                                                  ttCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTgcg                                                                            >  1:574617/1‑139 (MQ=255)                                                          ttATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGa                                                                    <  2:574617/139‑1 (MQ=255)                                                           tATCGAAATCCGTGAGCACTTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGAc                                                                   <  2:329412/139‑1 (MQ=255)                                                           tATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGAc                                                                   <  1:370003/139‑1 (MQ=255)                                                             tCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCt                                                                 >  1:472895/1‑139 (MQ=255)                                                                      gTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGa                                                        <  2:472895/139‑1 (MQ=255)                                                                       tGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTgg                                                               >  1:102376/1‑131 (MQ=255)                                                                       tGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTgg                                                               <  2:102376/131‑1 (MQ=255)                                                                         aGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAAt                                                     <  2:627793/139‑1 (MQ=255)                                                                            aCCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATccc                                                  <  1:102885/139‑1 (MQ=255)                                                                              cTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCgg                                                >  2:132051/1‑139 (MQ=255)                                                                                        aCGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTcgacg                                                                                                               >  1:597390/1‑66 (MQ=255)                                                                                        aCGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTcgacg                                                                                                               <  2:597390/66‑1 (MQ=255)                                                                                        aCGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCg                                                                                                      >  2:28184/1‑75 (MQ=255)                                                                                        aCGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCg                                                                                                      <  1:28184/75‑1 (MQ=255)                                                                                            cggcgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATc                                  >  1:118905/1‑139 (MQ=255)                                                                                             ggcgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCt                                 <  2:493628/139‑1 (MQ=255)                                                                                              gcgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCtt                                <  2:371221/139‑1 (MQ=255)                                                                                                   aaCATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGccc                           <  1:132051/139‑1 (MQ=255)                                                                                                            atcatcTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGcc                            <  2:346538/129‑1 (MQ=255)                                                                                                            atcatcTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGcc                            >  1:346538/1‑129 (MQ=255)                                                                                                            atcatcTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatga                  >  1:432454/1‑139 (MQ=255)                                                                                                               atcTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatgat                 >  2:89753/1‑137 (MQ=255)                                                                                                               atcTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatgat                 <  1:89753/137‑1 (MQ=255)                                                                                                                  tcCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCg            <  1:393934/139‑1 (MQ=255)                                                                                                                            aaaaCCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTAt  <  2:118905/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                               GTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTAT  >  NC_000913/1756239‑1756500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑