Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,904,586	T→C	V231A (GTC→GCC)	manZ →	mannose‑specific enzyme IID component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,904,586	0	T	C	100.0%	74.5 / NA	21	V231A (GTC→GCC)	manZ	mannose‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/12);  total (9/12)

CGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTA  >  NC_000913/1904469‑1904721                                                                                                                      |                                                                                                                                        cGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGtt                                                                                                                    <  1:326692/139‑1 (MQ=255)            ggCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGcc                                                                                                         <  2:187650/139‑1 (MQ=255)                                            gTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGTCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTAtg                                                                         >  2:680476/1‑139 (MQ=255)                                            gTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAgg                                                                                                           <  2:576054/105‑1 (MQ=255)                                            gTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAgg                                                                                                           >  1:576054/1‑105 (MQ=255)                                              ggACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTAtgtg                                                                       >  1:187710/1‑139 (MQ=255)                                               gACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGa                                                                                                                          <  1:357103/87‑1 (MQ=255)                                               gACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGa                                                                                                                          >  2:357103/1‑87 (MQ=255)                                                      gTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGAc                                                                                                                         <  2:458780/81‑1 (MQ=255)                                                      gTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGAc                                                                                                                         >  1:458780/1‑81 (MQ=255)                                                              cccGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGa                                                                                                                          >  1:156762/1‑72 (MQ=255)                                                              cccGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGa                                                                                                                          <  2:156762/72‑1 (MQ=255)                                                                       tGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGTCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCg                                              <  1:680476/139‑1 (MQ=255)                                                                                           aGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGa                                                                                                                          >  2:417254/1‑43 (MQ=255)                                                                                           aGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGa                                                                                                                          <  1:417254/43‑1 (MQ=255)                                                                                             aCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCt                                                                                    <  1:471715/79‑1 (MQ=255)                                                                                             aCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCt                                                                                    >  2:471715/1‑79 (MQ=255)                                                                                                ggCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGc                                                                                <  2:452860/80‑1 (MQ=255)                                                                                                ggCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGc                                                                                >  1:452860/1‑80 (MQ=255)                                                                                                    aaGAACACGTTACTACTGCCCAGACTATTCTGGGCCAGTTAATGCCAGGCCTGGTACCTCTGCGGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGAGCATCGTTGGCTTCTTCGTCAt                 <  1:15879/139‑1 (MQ=255)                                                                                                                   ctGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTa  <  2:187710/139‑1 (MQ=255)                                                                                                                      |                                                                                                                                        CGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTA  >  NC_000913/1904469‑1904721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑