Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,061,729 G→A R69W (CGG→TGG)  nac ← nitrogen assimilation regulon transcriptional regulator, autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,061,7290GA100.0% 61.2 / NA 19R69W (CGG→TGG) nacnitrogen assimilation regulon transcriptional regulator, autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/9);  total (10/9)

ATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGACCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAGTTGTTGATTTAACTCACCTTCCAGTGTGGCAACCTGCTGGCTGAGCGCTGGTTGTGCGATATGCAA  >  NC_000913/2061603‑2061861
                                                                                                                              |                                                                                                                                    
ataaGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGACCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGCATGGGtata                                                                                                                          <  2:48638/139‑1 (MQ=255)
        aTGGTGAAGGATGACGCAGCGGTTTCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGACCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCcacg                                                                                                                                    >  2:426745/1‑121 (MQ=255)
                                 cTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGACCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGcc                                                                                         <  1:55976/139‑1 (MQ=255)
                                        aaaGCCAATCGAGACTTGCCCCGATAATGCCTGACCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTa                                                                                              <  1:25756/127‑1 (MQ=255)
                                        aaaGCCAATCGAGACTTGCCCCGATAATGCCTGACCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTa                                                                                              >  2:25756/1‑127 (MQ=255)
                                        aaaGCCAATCGAGACTTGCCCCGATAATGCCTGACCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCttt                                                                                  >  2:429690/1‑139 (MQ=255)
                                                   agaCTTGCCCCGATAATGCCTGACCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCaa                                                                       <  1:433780/139‑1 (MQ=255)
                                                         gCCCCGATAATGCCTGACCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAgttg                                                                 >  2:12254/1‑139 (MQ=255)
                                                            ccGATAATGCCTGACCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAGttgttg                                                              <  1:429690/139‑1 (MQ=255)
                                                                    gCCTGACCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGTATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTa                                                                               <  1:236027/114‑1 (MQ=255)
                                                                    gCCTGACCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGTATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTa                                                                               >  2:236027/1‑114 (MQ=255)
                                                                         aCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGCATGGGTATAGAGTATTTTTCCGGCGTCTGTTTGTGTAACGCCCCGCTTTGTACGGATCAAAAGTGTTGATTTAACTCACCtt                                                >  1:404856/1‑139 (MQ=255)
                                                                         aCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGcc                                                                                         >  2:349971/1‑99 (MQ=255)
                                                                         aCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGcc                                                                                         <  1:349971/99‑1 (MQ=255)
                                                                         aCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAGTTGTTGATTTAACt                                                      >  1:527235/1‑134 (MQ=255)
                                                                         aCCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAGTTGTTGATTTAACt                                                      <  2:527235/134‑1 (MQ=255)
                                                                         aCCAACGTTATGCACCGCCAGTTGGGCCTGATCACACTGACGTAAAATGGCCCACGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAGTTGTTGATTTAACTCACCt                                                 >  1:601706/1‑139 (MQ=255)
                                                                                        cGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCACGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAGTTGTTGATTTAACTCACCTTCCAGTGTGGCAAcc                                  >  2:589422/1‑139 (MQ=255)
                                                                                                                          gCCCACGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAGGTTGTTGATTTAACTCACCTTCCAGTGTGGCAACCTGCTGGCTGAGCGCTGGTTGTGCGATATGCaa  >  2:405256/1‑137 (MQ=255)
                                                                                                                          gCCCACGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAGTTGTTGATTTAACTCACCTTCCAGTGTGGCAACCTGCTGGCTGAGCGCTGGTTGTGCGATATGCaa  <  1:405256/137‑1 (MQ=255)
                                                                                                                              |                                                                                                                                    
ATAAGGGCATGGTGATGGATGACGCAGCGGTTCCTGGTGCAAAGCCAATCGAGACTTGCCCCGATAATGCCTGACCAACGTTATGCACCGCCAGTTGGGCCTGTTCACACTGACGTAAAATGGCCCGCGCATGGGTATAGAGAATTTTTCCGGCGTCTGTTGGTGTAACGCCCCGCTTTGTACGGATCAAAAGTTGTTGATTTAACTCACCTTCCAGTGTGGCAACCTGCTGGCTGAGCGCTGGTTGTGCGATATGCAA  >  NC_000913/2061603‑2061861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: