Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,073,151 T→C L538P (CTG→CCG)  flu → CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,073,1510TC100.0% 78.2 / NA 21L538P (CTG→CCG) fluCP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/11);  total (10/11)

GGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACA  >  NC_000913/2073030‑2073286
                                                                                                                         |                                                                                                                                       
ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGgcagca                                                                                                                        <  1:355721/139‑1 (MQ=255)
ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGgcagca                                                                                                                        <  2:145743/139‑1 (MQ=255)
ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGgcagca                                                                                                                        <  1:97675/139‑1 (MQ=255)
           tGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAAc                                                                                                             >  2:16263/1‑139 (MQ=255)
                       cacaCTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCAtt                                                                                                    <  1:140073/136‑1 (MQ=255)
                       cacaCTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCAtt                                                                                                    >  2:140073/1‑136 (MQ=255)
                              aCCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGa                                                                                          >  2:383297/1‑139 (MQ=255)
                                    aaaGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCa                                                                                    >  2:314022/1‑139 (MQ=255)
                                                  gaatgaaGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGt                                                                      <  1:16263/139‑1 (MQ=255)
                                                         ggCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCt                                                               >  2:589665/1‑139 (MQ=255)
                                                             cgctgacgctgaACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGaa                                                           >  2:523009/1‑139 (MQ=255)
                                                                    gctgaACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTg                                                                                                         <  2:116776/86‑1 (MQ=255)
                                                                    gctgaACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTg                                                                                                         >  1:116776/1‑86 (MQ=255)
                                                                        aaCGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGt                                                                      <  2:315022/117‑1 (MQ=255)
                                                                        aaCGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGt                                                                      >  1:315022/1‑117 (MQ=255)
                                                                                tACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACg                                        >  1:81384/1‑139 (MQ=255)
                                                                                   cGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTg                                     <  1:523009/139‑1 (MQ=255)
                                                                                                     tGCTCAGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGAc                   <  2:81384/139‑1 (MQ=255)
                                                                                                          aGCGCGGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAg              <  1:589665/139‑1 (MQ=255)
                                                                                                            cgcgGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCAct                                                                                  >  1:531949/1‑69 (MQ=255)
                                                                                                            cgcgGTACAGCCCCGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCAct                                                                                  <  2:531949/69‑1 (MQ=255)
                                                                                                                      ccccGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACa  >  1:399102/1‑139 (MQ=255)
                                                                                                                         |                                                                                                                                       
GGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACA  >  NC_000913/2073030‑2073286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: