Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,092,066	G→A	G324S (GGC→AGC)	hisD →	bifunctional histidinal dehydrogenase/ histidinol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,092,066	0	G	A	100.0%	65.2 / NA	20	G324S (GGC→AGC)	hisD	bifunctional histidinal dehydrogenase/ histidinol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/10);  total (10/10)

CGTCGCGTTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAATCGGCAGGTGATTACGCCTC  >  NC_000913/2091931‑2092184                                                                                                                                        |                                                                                                                       cgtcgcgtTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGcc                                                                                                                     >  1:419815/1‑139 (MQ=255)                   gTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACTGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTc                                                                                                   >  1:517015/1‑139 (MQ=255)                             aaCTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCaa                                                                                         <  1:152021/139‑1 (MQ=255)                                 ggCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCATTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGcc                                                                                                                     >  1:36903/1‑107 (MQ=255)                                 ggCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCATTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGcc                                                                                                                     <  2:36903/107‑1 (MQ=255)                                              cgTGCCGAAACTGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCg                                                                        <  2:517015/139‑1 (MQ=255)                                                           cccgccAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGa                                                                                                         >  2:496910/1‑93 (MQ=255)                                                           cccgccAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGa                                                                                                         <  1:496910/93‑1 (MQ=255)                                                                                                  aaGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGt                                     <  1:455785/122‑1 (MQ=255)                                                                                                  aaGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGt                                     >  2:455785/1‑122 (MQ=255)                                                                                                   aGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCg                                                                        >  1:580889/1‑86 (MQ=255)                                                                                                   aGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCg                                                                        <  2:580889/86‑1 (MQ=255)                                                                                                   aGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGAt                                                                      >  1:485413/1‑88 (MQ=255)                                                                                                   aGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGAt                                                                      <  2:485413/88‑1 (MQ=255)                                                                                                        tAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAATCGGCAgg              >  1:444232/1‑139 (MQ=255)                                                                                                                tGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAAc                                                                              >  2:210746/1‑67 (MQ=255)                                                                                                                tGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAAc                                                                              <  1:210746/67‑1 (MQ=255)                                                                                                                    tGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAATCGGCAGGTGATTACGCCTc  <  2:168411/139‑1 (MQ=255)                                                                                                                            tcCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGt                                               >  2:229741/1‑86 (MQ=255)                                                                                                                            tcCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGt                                               <  1:229741/86‑1 (MQ=255)                                                                                                                                        |                                                                                                                       CGTCGCGTTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAATCGGCAGGTGATTACGCCTC  >  NC_000913/2091931‑2092184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑