Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,248,780	C→T	R280Q (CGG→CAG)	yeiE ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,248,780	0	C	T	100.0%	65.2 / NA	21	R280Q (CGG→CAG)	yeiE	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/11);  total (10/11)

GGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGAC  >  NC_000913/2248669‑2248908                                                                                                                |                                                                                                                                 ggCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCACGTTGGAAAGGTGTTTGTGcc                                                                                                       >  1:66467/1‑139 (MQ=255)     aaTGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTTCGGGATCGAAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                   <  1:80456/139‑1 (MQ=255)         catgcTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGt                                                                                               >  1:679204/1‑139 (MQ=255)             cTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATAc                                                                                           <  1:149684/139‑1 (MQ=255)                  ggCGCTGCGTCACGAAATCGACACACCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGTATCGAAATAGTCCAGACAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCcac                                                                                      >  1:383449/1‑139 (MQ=255)                          gTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACg                                                                              >  2:543345/1‑139 (MQ=255)                                 aaTCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAgg                                                                       <  1:543345/139‑1 (MQ=255)                                    cGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGgcgc                                                                    <  2:326272/139‑1 (MQ=255)                                                       tCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACtt                                                 <  2:383449/139‑1 (MQ=255)                                                            tACAGCAACTTAACGCGACACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACtt                                            >  1:399519/1‑139 (MQ=255)                                                                   aCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTa                                                                                                                               <  1:113101/49‑1 (MQ=255)                                                                   aCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTa                                                                                                                               >  2:113101/1‑49 (MQ=255)                                                                             gCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCca                                                                                       >  1:586090/1‑79 (MQ=255)                                                                             gCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCca                                                                                       <  2:586090/79‑1 (MQ=255)                                                                                         gATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAAt               <  2:679204/139‑1 (MQ=255)                                                                                           tCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGgcgc                                                                    >  1:399628/1‑84 (MQ=255)                                                                                           tCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGgcgc                                                                    <  2:399628/84‑1 (MQ=255)                                                                                                    tCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGc                                                                                                        >  2:102843/1‑39 (MQ=255)                                                                                                    tCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGc                                                                                                        <  1:102843/39‑1 (MQ=255)                                                                                                      cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGAc  >  1:214325/1‑139 (MQ=255)                                                                                                             cgcTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTc                                                <  2:213895/86‑1 (MQ=255)                                                                                                                |                                                                                                                                 GGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGAC  >  NC_000913/2248669‑2248908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑