Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,250,031 C→T I105I (ATC→ATT yeiH → UPF0324 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,250,0310CT100.0% 95.7 / NA 28I105I (ATC→ATTyeiHUPF0324 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/13);  total (15/13)

CGCACATCTGGAAAAGCTGTGACGGTGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTA  >  NC_000913/2249904‑2250163
                                                                                                                               |                                                                                                                                    
cGCACATCTGGAAAAGCTGTGACGGTGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACgt                                                                                                                           >  1:456404/1‑139 (MQ=255)
       cTGGAAAAGCTGTGACGGTGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACg                                                                                                                    <  1:273165/139‑1 (MQ=255)
       cTGGAAAAGCTGTGACGGTGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACg                                                                                                                    <  2:226/139‑1 (MQ=255)
        tGGAAAAGCTGTGACGGTGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACGc                                                                                                                   <  2:456404/139‑1 (MQ=255)
                                 tGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGgcttg                                                                                          <  1:43903/139‑1 (MQ=255)
                                                          tCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACgtgt                                                                                                                         <  2:31986/83‑1 (MQ=255)
                                                          tCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACgtgt                                                                                                                         >  1:31986/1‑83 (MQ=255)
                                                           cTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTc                                                                >  2:231631/1‑139 (MQ=255)
                                                           cTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTc                                                                >  1:604507/1‑139 (MQ=255)
                                                                        tttATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATa                                                          >  1:525346/1‑132 (MQ=255)
                                                                        tttATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATa                                                          <  2:525346/132‑1 (MQ=255)
                                                                         ttATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACg                                                                                                                    <  1:152198/73‑1 (MQ=255)
                                                                         ttATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACg                                                                                                                    >  2:152198/1‑73 (MQ=255)
                                                                             ggCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAg                                                                             <  2:342236/108‑1 (MQ=255)
                                                                             ggCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAg                                                                             >  1:342236/1‑108 (MQ=255)
                                                                                              ctcGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGt                             >  1:181212/1‑139 (MQ=255)
                                                                                               tcGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACGCTGTCCa                                                                                                             >  1:327941/1‑58 (MQ=255)
                                                                                               tcGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACGCTGTCCa                                                                                                             <  2:327941/58‑1 (MQ=255)
                                                                                               tcGCAAATTGCCGATGTCGGTATCAGTGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTa                            >  2:51314/1‑139 (MQ=255)
                                                                                                             tGTCGGTATCAGTGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGgcttg                                                                                          <  2:658691/63‑1 (MQ=255)
                                                                                                             tGTCGGTATCAGTGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGgcttg                                                                                          >  1:658691/1‑63 (MQ=255)
                                                                                                               tCGGTATCAGTGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGtgc            <  2:604507/139‑1 (MQ=255)
                                                                                                                        gTGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGt   >  2:601977/1‑139 (MQ=255)
                                                                                                                         tGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATa                                                          >  1:571062/1‑83 (MQ=255)
                                                                                                                         tGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATa                                                          <  2:571062/83‑1 (MQ=255)
                                                                                                                         tGGGATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTa  >  2:204509/1‑139 (MQ=255)
                                                                                                                           ggATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGt                                           <  2:525943/96‑1 (MQ=255)
                                                                                                                           ggATTATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGt                                           >  1:525943/1‑96 (MQ=255)
                                                                                                                               |                                                                                                                                    
CGCACATCTGGAAAAGCTGTGACGGTGGTGTGCTGTTTGCCAAGCAATATTTATTACGTCTGGGTATCATTCTTTATGGCTTCCGTCTGACGTTCTCGCAAATTGCCGATGTCGGTATCAGTGGGATCATCATTGACGTGTTGACGCTGTCCAGTACCTTCCTGCTGGCTTGCTTCCTGGGGCAGAAAGTGTTTGGTCTGGATAAGCACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTA  >  NC_000913/2249904‑2250163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: