| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 350,431 | G→A | L140L (CTG→CTA) | prpC → | 2‑methylcitrate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 350,431 | 0 | G | A | 100.0% | 99.1 / NA | 29 | L140L (CTG→CTA) | prpC | 2‑methylcitrate synthase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/14); total (15/14) | |||||||||||
CTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCA > NC_000913/350300‑350535 | cTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGc > 2:590723/1‑139 (MQ=255)cTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGc > 2:127866/1‑139 (MQ=255) aaGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAg < 2:617414/139‑1 (MQ=255) tACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGAt > 1:296703/1‑139 (MQ=255) ccGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATtc < 2:296703/139‑1 (MQ=255) cGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGAtt > 1:223697/1‑103 (MQ=255) cGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGAtt < 2:223697/103‑1 (MQ=255) cGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGaa > 1:316572/1‑139 (MQ=255) ccGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGAt < 1:157481/94‑1 (MQ=255) ccGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGAt > 2:157481/1‑94 (MQ=255) gcgcTCGGCTGCACGCTGCCATAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAATTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAAc < 1:407506/139‑1 (MQ=255) cGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCcaca > 1:600698/1‑116 (MQ=255) cGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCcaca < 2:600698/116‑1 (MQ=255) gagGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCGTcggcg < 1:590723/139‑1 (MQ=255) gagGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATcggcg < 1:199612/139‑1 (MQ=255) agGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACt < 1:226925/84‑1 (MQ=255) agGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACt > 2:226925/1‑84 (MQ=255) agGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCAt < 1:43329/133‑1 (MQ=255) agGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCAt > 2:43329/1‑133 (MQ=255) gCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTAtt > 1:382654/1‑71 (MQ=255) gCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTAtt < 2:382654/71‑1 (MQ=255) tctcTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCa > 2:633758/1‑139 (MQ=255) tctcTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGACACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCa > 1:499996/1‑139 (MQ=255) cgGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATc > 2:655190/1‑58 (MQ=255) cgGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATc < 1:655190/58‑1 (MQ=255) cgGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCa < 2:10658/119‑1 (MQ=255) cgGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCa > 1:10658/1‑119 (MQ=255) tGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCa > 2:529999/1‑78 (MQ=255) tGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCa < 1:529999/78‑1 (MQ=255) | CTGGAAGCCTTACCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCA > NC_000913/350300‑350535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |