Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,504,660	G→A	P296P (CCC→CCT)	ypdE ←	aminopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,504,660	0	G	A	100.0%	22.2 / NA	8	P296P (CCC→CCT)	ypdE	aminopeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/3);  total (5/3)

GATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTGGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTGCCGCCGTTGCTGAACATATCTGCCTGCAACGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAA  >  NC_000913/2504568‑2504775                                                                                             |                                                                                                                    gATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTAGGCACgccgg                                                                                                           <  1:352244/103‑1 (MQ=255) gATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTAGGCACgccgg                                                                                                           >  2:352244/1‑103 (MQ=255) gATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTAGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTgccgc                                                                       >  2:317423/1‑139 (MQ=255)                                  caatcggcaatcgATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTAGGCACGCCGGTGCCGGTTAAGTg                                                                                              <  1:218678/83‑1 (MQ=255)                                  caatcggcaatcgATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTAGGCACGCCGGTGCCGGTTAAGTg                                                                                              >  2:218678/1‑83 (MQ=255)                                                  cggcgCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTAGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTGCCGCCGTTGCTGAACATATCTGCCTGCAACGGCACGCCAATTTCTGCCGCCAc                      <  1:317423/139‑1 (MQ=255)                                                          tGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTAGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTGCCGCCGTTGCTGAACATATCTGCCTGCAACGGCACGCCAATTTCTGCCGCCACGGTTTCGa              >  2:204893/1‑139 (MQ=255)                                                                      gTTGCTGGCCCCATCACCACTGTAGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTGCCGCCGTTGCTGAACATATCTGCCTGCAACGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTaa  >  1:167904/1‑139 (MQ=255)                                                                                             |                                                                                                                    GATAAAAGTTGCTGCATCTGCAAAATGTCGCGGCAATCGGCAATCGATGCGGCGCAATGTCCATGGCGGGTTGCTGGCCCCATCACCACTGTGGGCACGCCGGTGCCGGTTAAGTGCACCGCCCCGCCGTCCGTGCCGCCGTTGCTGAACATATCTGCCTGCAACGGCACGCCAATTTCTGCCGCCACGGTTTCGACCCAGGCGGTAA  >  NC_000913/2504568‑2504775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑