Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,505,003	T→C	H182R (CAC→CGC)	ypdE ←	aminopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,505,003	0	T	C	100.0%	41.3 / NA	12	H182R (CAC→CGC)	ypdE	aminopeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/5);  total (7/5)

GCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCT  >  NC_000913/2504908‑2505138                                                                                                |                                                                                                                                         gCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCg                                                                                              <  1:157363/139‑1 (MQ=255)      acCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCCAACCGAGGCGGtca                                                                                          >  2:454375/1‑139 (MQ=255)             gggTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCCAACCGAGGCGGTCATCAAAgg                                                                                   <  1:454375/139‑1 (MQ=255)                         gCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCg                                                                                              <  1:176154/115‑1 (MQ=255)                         gCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCg                                                                                              >  2:176154/1‑115 (MQ=255)                              ccgcGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGtcatc                                                                                        <  1:626018/116‑1 (MQ=255)                              ccgcGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGtcatc                                                                                        >  2:626018/1‑116 (MQ=255)                                            ctctTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAAcc                                                   >  1:566293/1‑139 (MQ=255)                                                 cGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGaaa                                              >  1:549194/1‑139 (MQ=255)                                                         cTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTAt                                      >  2:643631/1‑139 (MQ=255)                                                                           tCCGCAGGTAGTTCGGTGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGc                    >  2:637017/1‑139 (MQ=255)                                                                                         ggCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTccc      <  2:258210/139‑1 (MQ=255)                                                                                             tcgcgcagttcgcgcagtAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCt  <  2:549194/139‑1 (MQ=255)                                                                                                |                                                                                                                                         GCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAG‑CAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATTCCCGCCT  >  NC_000913/2504908‑2505138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑