Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,706,066	T→C	E353E (GAA→GAG)	lepA ←	back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,706,066	0	T	C	100.0%	61.1 / NA	17	E353E (GAA→GAG)	lepA	back‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

GATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAA  >  NC_000913/2705937‑2706197                                                                                                                                  |                                                                                                                                    gATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGgatg                                                                                                                            <  1:512971/139‑1 (MQ=255)                               gCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCg                                                                                              <  1:556828/139‑1 (MQ=255)                                                      ttGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAgcgc                                                                       >  1:172521/1‑139 (MQ=255)                                                                      ttCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCGGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGc                                                       <  1:59566/139‑1 (MQ=255)                                                                               tACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAAc                                              >  2:77342/1‑139 (MQ=255)                                                                               tACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAAc                                              >  1:195142/1‑139 (MQ=255)                                                                                    tCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGa                                         >  2:373314/1‑139 (MQ=255)                                                                                       gcgcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGc                                      <  1:373314/139‑1 (MQ=255)                                                                                                  aTCAGATCCAGATCGTATTCACGTTCCAGACGCTcc                                                                                                                                  <  1:231757/36‑1 (MQ=255)                                                                                                  aTCAGATCCAGATCGTATTCACGTTCCAGACGCTcc                                                                                                                                  >  2:231757/1‑36 (MQ=255)                                                                                                  aTCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGc                           <  1:77342/139‑1 (MQ=255)                                                                                                         ccagatcGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGc                                      >  1:187486/1‑121 (MQ=255)                                                                                                         ccagatcGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGc                                      <  2:187486/121‑1 (MQ=255)                                                                                                            gatcGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGa                                                                                       >  1:155806/1‑69 (MQ=255)                                                                                                            gatcGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGa                                                                                       <  2:155806/69‑1 (MQ=255)                                                                                                                 tATTCAGGTTCCAGATTCTCCCGGATGATCGCCATTGGTAGCAGGAAGGGGAAGCCGCATCGGAAACCCAAGCCCAGCGCGCTGAAGCTTTCCGGCTCCTAGAACAGTGAGGCATCGTTCAGGCTCAGTTTACCCAgcg            >  1:394460/1‑139 (MQ=255)                                                                                                                         ttCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACgg    >  1:103733/1‑139 (MQ=255)                                                                                                                           ccAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGaa  <  2:195142/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                    GATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAA  >  NC_000913/2705937‑2706197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑