Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,733,223 G→A D317D (GAC→GAT clpB ← protein disaggregation chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,733,2230GA100.0% 99.1 / NA 29D317D (GAC→GATclpBprotein disaggregation chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/11);  total (18/11)

TGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGAC  >  NC_000913/2733095‑2733357
                                                                                                                                |                                                                                                                                      
tgCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGcgtcg                                                                                                                              >  1:366838/1‑139 (MQ=255)
       cGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCAcc                                                                                                                       >  1:265746/1‑139 (MQ=255)
          aaCGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCGTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTAc                                                                                                                    >  1:155404/1‑139 (MQ=255)
           aCGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACg                                                                                                                   <  2:366838/139‑1 (MQ=255)
                                aaTCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTgg                                                                                                                           >  2:605346/1‑110 (MQ=255)
                                aaTCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTgg                                                                                                                           <  1:605346/110‑1 (MQ=255)
                                     cAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAgcgc                                                                                         <  1:327203/139‑1 (MQ=255)
                                            aTCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTc                                                                                  <  2:120364/139‑1 (MQ=255)
                                                    cAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGtt                                                                          >  2:694166/1‑139 (MQ=255)
                                                       aaGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGAGCCAGCGCCGGTTTCAGCATGTTTcc                                                                       >  1:444666/1‑139 (MQ=255)
                                                          gCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAATCGTCGTGGCACCTACCCAGTGCAATTAACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGc                                                                    <  2:444666/139‑1 (MQ=255)
                                                              ggCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTcc                                                                >  1:66232/1‑139 (MQ=255)
                                                                       cacTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCa                                                       <  2:265746/139‑1 (MQ=255)
                                                                          tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCa                                                                                             <  1:148861/98‑1 (MQ=255)
                                                                          tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCa                                                                                             >  2:148861/1‑98 (MQ=255)
                                                                            tCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGc                                                  >  2:411471/1‑139 (MQ=255)
                                                                                    cgacgTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCcg                                          >  2:239335/1‑139 (MQ=255)
                                                                                         ctCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCg                                     >  1:517128/2‑139 (MQ=255)
                                                                                          tCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGa                                    >  1:35412/1‑139 (MQ=255)
                                                                                                  agcaTCTTTTTCAATGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTa                            <  2:155404/139‑1 (MQ=255)
                                                                                                                tGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATtcgtcg                <  2:218894/137‑1 (MQ=255)
                                                                                                                tGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATtcgtcg                >  1:218894/1‑137 (MQ=255)
                                                                                                                tGTACTGGCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGAATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGAt              >  2:664367/1‑139 (MQ=255)
                                                                                                                       gCGATATTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAgg       >  1:291490/1‑139 (MQ=255)
                                                                                                                          atatTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTAcc                                            >  1:649710/1‑99 (MQ=255)
                                                                                                                          atatTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTAcc                                            <  2:649710/99‑1 (MQ=255)
                                                                                                                            atTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGttt                                                                                   >  1:613224/1‑58 (MQ=255)
                                                                                                                            atTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGttt                                                                                   <  2:613224/58‑1 (MQ=255)
                                                                                                                            atTCATCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGAc  >  1:211309/1‑139 (MQ=255)
                                                                                                                                |                                                                                                                                      
TGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGTTTCCGGCGTCCATTGCGCCATCGGCTTTACCCGCGCCGACCATGGTATGTAATTCGTCGATAAATAGGATGAC  >  NC_000913/2733095‑2733357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: