Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,744,303	A→G	T76T (ACT→ACC)	rplS ←	50S ribosomal subunit protein L19

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,744,303	0	A	G	100.0%	68.8 / NA	19	T76T (ACT→ACC)	rplS	50S ribosomal subunit protein L19
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/10);  total (9/10)

AATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCTTCAA  >  NC_000913/2744179‑2744433                                                                                                                             |                                                                                                                                   aaTCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACg                                                                                                                      >  1:54548/1‑139 (MQ=255)                                  ccTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAAc                                                                                     <  1:191/139‑1 (MQ=255)                                         aGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAAt                                                                              <  1:243570/139‑1 (MQ=255)                                            aCGCTCACGCAGGTAGTACAGTTTAGCTTTACGAGCAGCACCACGACGTTTAACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCa                                                                                                                  >  1:430954/1‑100 (MQ=255)                                            aCGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCa                                                                           >  1:96883/1‑139 (MQ=255)                                            aCGCTCACGCAGGTAGTACAGTATAGCTTTACGAGCAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCa                                                                                                                  <  2:430954/100‑1 (MQ=255)                                                tCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGt                                                                       >  1:361322/1‑139 (MQ=255)                                                        gtagtaAAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACcg                                                               >  2:248120/1‑139 (MQ=255)                                                            taCAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGt                                                           >  2:361903/1‑139 (MQ=255)                                                                 tttAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGa                                                      >  2:140700/1‑139 (MQ=255)                                                                  ttAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGaa                                                     <  2:54548/139‑1 (MQ=255)                                                                      cTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGc                                                 >  1:379878/1‑139 (MQ=255)                                                                                aGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACg                                       <  2:549908/139‑1 (MQ=255)                                                                                                  aGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGa                     <  2:379878/139‑1 (MQ=255)                                                                                                      aTGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGtt                 >  2:316291/1‑139 (MQ=255)                                                                                                           gTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTg            <  1:245336/139‑1 (MQ=255)                                                                                                             cAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGa          <  1:361903/139‑1 (MQ=255)                                                                                                                     ggAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCTTcaa  <  1:316291/139‑1 (MQ=255)                                                                                                                     ggAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCTTcaa  <  2:96883/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                   AATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCTTCAA  >  NC_000913/2744179‑2744433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑