Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,759,929 T→C H315H (CAT→CAC yfjI → CP4‑57 prophage, uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,759,9290TC100.0% 52.3 / NA 15H315H (CAT→CACyfjICP4‑57 prophage, uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/7);  total (8/7)

AATTTTTTCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGC  >  NC_000913/2759803‑2760027
                                                                                                                              |                                                                                                  
aaTTTTTTCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACACTGGATCGAATTc                                                                                        <  2:48026/139‑1 (MQ=255)
                                                   tGTTAATGAGAGCATCATTAAAATTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACACTGGATCg                                                                                             <  1:472043/83‑1 (MQ=255)
                                                   tGTTAATGAGAGCATCATTAAAATTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACACTGGATCg                                                                                             >  2:472043/1‑83 (MQ=255)
                                                     ttAATGAGAGCATCATTAAAATTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACACTGGATCGAATTCTACAACCAGGt                                                                             >  1:56165/1‑97 (MQ=255)
                                                     ttAATGAGAGCATCATTAAAATTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACACTGGATCGAATTCTACAACCAGGt                                                                             <  2:56165/97‑1 (MQ=255)
                                                           agagCATCATTAAAATTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACACTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTa                             <  2:333248/139‑1 (MQ=255)
                                                             agCATCATTAAAATTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACACTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGc                                                    <  1:28458/114‑1 (MQ=255)
                                                             agCATCATTAAAATTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACACTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGc                                                    >  2:28458/1‑114 (MQ=255)
                                                             agCATCATTAAAATTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACACTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATg                           >  2:128072/1‑139 (MQ=255)
                                                             agCATCATTAAAATTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACACTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATg                           >  2:479113/1‑139 (MQ=255)
                                                               catcatTAAAATTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACACTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCt                         >  1:166500/1‑139 (MQ=255)
                                                                       aaaTTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACACTGGATCGa                                                                                            <  1:536393/64‑1 (MQ=255)
                                                                       aaaTTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACACTGGATCGa                                                                                            >  2:536393/1‑64 (MQ=255)
                                                                       aaaTTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACACTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAAt                 >  1:179589/1‑139 (MQ=255)
                                                                                      atCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACACTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGc  <  2:179589/139‑1 (MQ=17)
                                                                                                                              |                                                                                                  
AATTTTTTCAAATGAACATTTGCCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATTAATGAAAATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACATTGGATCGAATTCTACAACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCTTCTAAAATGGCGGAGAACATGGC  >  NC_000913/2759803‑2760027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: