Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,032,253	G→A	R296H (CGT→CAT)	uacT →	uric acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,032,253	0	G	A	100.0%	143.6 / NA	42	R296H (CGT→CAT)	uacT	uric acid permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/20);  total (22/20)

TCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATTTCTTCGGGAATTA  >  NC_000913/3032121‑3032384                                                                                                                                     |                                                                                                                                    tCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGa                                                                                                                               <  1:179361/139‑1 (MQ=255)        cGTTTCCATTCTGACCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTa                                                                                                                        <  2:120641/139‑1 (MQ=255)         gTTTCCATTCTGACCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAg                                                                                                                       >  2:205927/1‑139 (MQ=255)            tCCATTCTGACCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGa                                                                                                                    <  1:352821/139‑1 (MQ=255)                     aCCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTa                                                                                                                        <  1:383335/126‑1 (MQ=255)                     aCCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTa                                                                                                                        >  2:383335/1‑126 (MQ=255)                     aCCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGa                                                                                                                    <  2:199758/130‑1 (MQ=255)                     aCCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGa                                                                                                                    >  1:199758/1‑130 (MQ=255)                           aCTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCa                                                                                                     >  2:22853/1‑139 (MQ=255)                              gCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGt                                                                                                  >  2:113886/1‑139 (MQ=255)                                                tttATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCcaca                                                                                >  2:130773/1‑139 (MQ=255)                                                         tCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTa                                                                                                                        <  1:303392/90‑1 (MQ=255)                                                         tCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTa                                                                                                                        >  2:303392/1‑90 (MQ=255)                                                                  aTGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACg                                                              <  1:205927/139‑1 (MQ=255)                                                                  aTGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACg                                                              <  2:598996/139‑1 (MQ=255)                                                                       ccTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGc                                                         >  2:529920/1‑139 (MQ=255)                                                                       ccTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGc                                                         <  1:22853/139‑1 (MQ=255)                                                                       ccTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGc                                                         <  2:243264/139‑1 (MQ=255)                                                                         tGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCt                                                       >  2:206832/1‑139 (MQ=255)                                                                          ggCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTg                                                      >  2:545108/1‑139 (MQ=255)                                                                                 ggTGTAATAGTCGGTCGTAAAGTCTCTTCGCAAGCTATTATTCATGGGCTGAATGTCGGTCGCGCATGGACAATGATAGACGACCCGTATAACCGCTCCCCCCACCATTCATTTCATGAAAAGCTTGACCTGGATaacc                                               >  1:324819/1‑136 (MQ=255)                                                                                 ggTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCg                                               >  2:94883/1‑139 (MQ=255)                                                                                 ggTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCg                                               >  2:572711/1‑139 (MQ=255)                                                                                 ggTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCg                                               >  2:404227/1‑139 (MQ=255)                                                                                 ggTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCg                                               >  2:17428/1‑139 (MQ=255)                                                                                  gTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGt                                              >  2:208646/1‑139 (MQ=255)                                                                                           tcggtcgTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGt                                     >  2:599759/1‑139 (MQ=255)                                                                                                cgTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATa                                >  1:677478/1‑139 (MQ=255)                                                                                                      ctctctTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCt                          <  1:113886/139‑1 (MQ=255)                                                                                                      ctctctTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCt                          <  1:17428/139‑1 (MQ=255)                                                                                                      ctctctTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCt                          <  1:529920/139‑1 (MQ=255)                                                                                                        ctctTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGt                                                                                                                         <  1:475307/42‑1 (MQ=255)                                                                                                        ctctTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGt                                                                                                                         >  2:475307/1‑42 (MQ=255)                                                                                                        ctctTCGCACGATATTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTgg                        <  1:208646/139‑1 (MQ=255)                                                                                                                   atatTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTgg                        <  1:228044/128‑1 (MQ=255)                                                                                                                   atatTATTCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTgg                        >  2:228044/1‑128 (MQ=255)                                                                                                                       tattCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATTTCTTCg         <  1:206832/139‑1 (MQ=255)                                                                                                                        attCGCGGGCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATTTCTTCgg        <  1:130773/139‑1 (MQ=255)                                                                                                                              gggCTGCATGTCGATGGCGTAGGGACAATGATCGGCGGCACGTTTaa                                                                                              >  2:603944/1‑47 (MQ=255)                                                                                                                              gggCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTaa                                                                                              <  1:603944/47‑1 (MQ=255)                                                                                                                              gggCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATTTCTTCGGGAAt    >  2:353513/1‑137 (MQ=255)                                                                                                                              gggCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATTTCTTCGGGAAt    <  1:353513/137‑1 (MQ=255)                                                                                                                              gggCTGCATGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATTTCTTCGGGAATta  >  2:354240/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                    TCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAATCATCGTGTTTATCGAGTCAATGGGGATGTTCCTGGCACTGGGTGAAATAGTCGGTCGTAAACTCTCTTCGCACGATATTATTCGCGGGCTGCGTGTCGATGGCGTAGGGACAATGATAGGCGGCACGTTTAACAGCTTCCCCCACACGTCATTTTCTCAAAACGTTGGCCTGGTTAGCGTGACGCGCGTTCATAGCCGCTGGGTGTGTATTTCTTCGGGAATTA  >  NC_000913/3032121‑3032384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑