Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,306,716	C→T	R382H (CGC→CAC)	deaD ←	ATP‑dependent RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,306,716	0	C	T	100.0%	92.6 / NA	28	R382H (CGC→CAC)	deaD	ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/14);  total (14/14)

CCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGCGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGA  >  NC_000913/3306603‑3306850                                                                                                                  |                                                                                                                                       ccAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCgg                                                                                                               <  1:552761/139‑1 (MQ=255)  cAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGc                                                                                                              >  1:609866/1‑139 (MQ=255)       ctTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTc                                                                                                         <  1:265408/139‑1 (MQ=255)        ttCACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCt                                                                                                        <  2:37393/139‑1 (MQ=255)          cACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTAc                                                                                                      >  2:213026/1‑139 (MQ=255)            ccTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACtt                                                                                                    <  1:213026/139‑1 (MQ=255)                      tCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGt                                                                                          >  1:77114/1‑139 (MQ=255)                         gCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAg                                                                                       <  2:609866/139‑1 (MQ=255)                                  tGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGt                                                                              <  2:77114/139‑1 (MQ=255)                                                             gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGt                                                                                          >  1:286877/1‑100 (MQ=255)                                                             gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGt                                                                                          <  2:286877/100‑1 (MQ=255)                                                             gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTgcgc                                                              >  2:78505/1‑128 (MQ=255)                                                             gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTgcgc                                                              <  1:78505/128‑1 (MQ=255)                                                             gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCgcag                                                            <  2:699710/130‑1 (MQ=255)                                                             gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCgcag                                                            >  1:699710/1‑130 (MQ=255)                                                             gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGacgacg                                                   >  1:293157/1‑139 (MQ=255)                                                             gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGacgacg                                                   >  2:512216/1‑139 (MQ=255)                                                             gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGacgacg                                                   >  2:493093/1‑139 (MQ=255)                                                                   tgctTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGATGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAgac                                                       <  1:66331/129‑1 (MQ=255)                                                                   tgctTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAgac                                                       >  2:66331/1‑129 (MQ=255)                                                                        tCCAGCTGCTGCTGTACTTTAGCGGCGAA‑TTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACg                                                                           <  2:644106/103‑1 (MQ=255)                                                                        tCCAGCTGCTGCTGTACTTTAGCGGCGAA‑TTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACg                                                                           >  1:644106/1‑103 (MQ=255)                                                                          cAGCTGCTCCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCa                                      >  2:625919/1‑139 (MQ=255)                                                                                           tAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGcgcgcg                     >  1:491906/1‑139 (MQ=255)                                                                                              cggcgAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTAc                                                                                                      >  1:193602/1‑55 (MQ=255)                                                                                              cggcgAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTAc                                                                                                      <  2:193602/55‑1 (MQ=255)                                                                                                           cAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCa     <  2:293157/139‑1 (MQ=255)                                                                                                              aCGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGa  <  2:491906/139‑1 (MQ=255)                                                                                                                  |                                                                                                                                       CCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGCGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGA  >  NC_000913/3306603‑3306850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑