Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,349,840	T→C	K207R (AAG→AGG)	elbB ←	isoprenoid biosynthesis protein with amidotransferase‑like domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,349,840	0	T	C	100.0%	67.5 / NA	19	K207R (AAG→AGG)	elbB	isoprenoid biosynthesis protein with amidotransferase‑like domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/12);  total (7/12)

CCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCACATGCTCCGCGCCCATC  >  NC_000913/3349727‑3349965                                                                                                                  |                                                                                                                              ccccAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGc                                                                                                      >  2:622903/1‑139 (MQ=255)  cccAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCa                                                                                                     <  1:622903/139‑1 (MQ=255)       aaaaCGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGtt                                                                                                <  1:434591/139‑1 (MQ=255)            ggCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTgcg                                                                                           >  2:287163/1‑139 (MQ=255)               gAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCa                                                                                        <  2:419191/139‑1 (MQ=255)                   aCAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCat                                                                                    <  2:280151/139‑1 (MQ=255)                          ttAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCt                                                                             <  2:283791/139‑1 (MQ=255)                                 cAAAAGAAAACGGCGAACGAAACTAAAAACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGACTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGggtgg                                                                      >  1:171739/1‑139 (MQ=255)                                  aaaaGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGggtggt                                                                     <  2:171739/139‑1 (MQ=255)                                  aaaaGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGggtggt                                                                     <  1:287163/139‑1 (MQ=255)                                                               cGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCatata                                                                                 <  1:509909/98‑1 (MQ=255)                                                               cGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCatata                                                                                 >  2:509909/1‑98 (MQ=255)                                                               cGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTAGCCGCTTCTGCAATGTTCTTCGCCAGCATATACGCTGGGGTGGTGTGAATCTTATTGTCTTCATCAACCACGa                                        >  1:183256/1‑139 (MQ=255)                                                                         cTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCACTcgccg                                                                                                             <  2:457202/60‑1 (MQ=255)                                                                         cTTTTACCCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCACTcgccg                                                                                                             >  1:457202/1‑60 (MQ=255)                                                                          ttttACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACa                             <  1:565349/139‑1 (MQ=255)                                                                                            aaCCAGCACGCGGGAAACCAGCCTATCAATGCCGCTcgc                                                                                                               >  1:621077/1‑39 (MQ=255)                                                                                            aaCCAGCACGCGGGAAACCAGCCTATCAATGCCGCTcgc                                                                                                               <  2:621077/39‑1 (MQ=255)                                                                                                     gcgGGAAACCAGCCTCTCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTACTGTCTTCATCAACCACGATATCATCGACAGGACACGGCACATGCTCCGCGCCCAtc  <  2:183256/139‑1 (MQ=255)                                                                                                                  |                                                                                                                              CCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGACAGGACACGGCACATGCTCCGCGCCCATC  >  NC_000913/3349727‑3349965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑