Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,936,381 C→T T35M (ACG→ATG)  rbsB → D‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,936,3810CT100.0% 145.5 / NA 43T35M (ACG→ATG) rbsBD‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/26);  total (17/26)

ACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGAAAGAGCTGGCGAACGTGCAGGA  >  NC_000913/3936259‑3936507
                                                                                                                          |                                                                                                                              
aCTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTc                                                                                                                >  2:579413/1‑139 (MQ=255)
    cAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTg                                                                                                            <  2:355557/139‑1 (MQ=255)
      ggACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTa                                                                                                          <  1:579413/139‑1 (MQ=255)
         cATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCg                                                                                                       >  1:577798/1‑139 (MQ=255)
         cATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCg                                                                                                       <  2:577798/139‑1 (MQ=255)
         cATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCg                                                                                                       >  2:507317/1‑139 (MQ=255)
          aTCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGtt                                                                                                                 <  1:7216/128‑1 (MQ=255)
          aTCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGtt                                                                                                                 >  2:7216/1‑128 (MQ=255)
            cTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTg                                                                                                    >  2:556911/1‑139 (MQ=255)
            cTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTg                                                                                                    >  2:10466/1‑139 (MQ=255)
                 atatGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAAccc                                                                                                                    <  2:594958/118‑1 (MQ=255)
                 atatGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAAccc                                                                                                                    >  1:594958/1‑118 (MQ=255)
                    tGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTGAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATgg                                                                                            <  2:270788/139‑1 (MQ=255)
                        cATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCGATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcgcg                                                                                        <  2:514968/139‑1 (MQ=255)
                           gAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTaacaac                                                                                                                      >  2:530595/1‑106 (MQ=255)
                           gAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTaacaac                                                                                                                      <  1:530595/106‑1 (MQ=255)
                              aaaaCTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGaaa                                                                                  <  1:507317/139‑1 (MQ=255)
                                       tACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGTGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGAt                                                                         <  2:158334/139‑1 (MQ=255)
                                            tGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACt                                                                    <  1:10466/139‑1 (MQ=255)
                                                 tCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCt                                                               <  2:372419/139‑1 (MQ=255)
                                                    gCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCtata                                                            >  2:323714/1‑139 (MQ=255)
                                                          gcgcTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCtgg                                                      <  2:551252/139‑1 (MQ=255)
                                                                aGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGc                                                <  2:190382/139‑1 (MQ=255)
                                                                  cgcCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTg                                              >  1:671481/1‑139 (MQ=255)
                                                                   gcCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTgg                                             <  1:323714/139‑1 (MQ=255)
                                                                   gcCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTgg                                             <  1:556911/139‑1 (MQ=255)
                                                                                 gAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTaacaac                                                                                                                      <  2:415649/52‑1 (MQ=255)
                                                                                 gAATGCGATGGCAAAAGACACCATCGCGCTGGAGGTCTCCATGCTTaacaac                                                                                                                      >  1:415649/1‑52 (MQ=255)
                                                                                        aTGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGa                        >  1:355383/1‑139 (MQ=255)
                                                                                          ggCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGaaa                      <  1:365013/139‑1 (MQ=255)
                                                                                                   cacCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGa                                                                                               >  2:323491/1‑57 (MQ=255)
                                                                                                   cacCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGa                                                                                               <  1:323491/57‑1 (MQ=255)
                                                                                                      cATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTgg                                             >  1:609976/1‑104 (MQ=255)
                                                                                                      cATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTgg                                             <  2:609976/104‑1 (MQ=255)
                                                                                                        tCGCGCTGGTGGTCTCCATGCTTAAGAACCCGTTCTTTGTa                                                                                                          <  1:467703/41‑1 (MQ=38)
                                                                                                        tCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTCGTa                                                                                                          >  2:467703/1‑41 (MQ=255)
                                                                                                         cgcgCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACCCCCAGAACAACCCGGCGAAAGAGCTGGCGAACGTg       <  2:257753/139‑1 (MQ=255)
                                                                                                         cgcgCTGGTAGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGAAAGAGCTGGCGAACGTg       <  1:480486/139‑1 (MQ=255)
                                                                                                             cTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTAt                                                                                                         <  1:104304/37‑1 (MQ=255)
                                                                                                             cTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTAt                                                                                                         >  2:104304/1‑37 (MQ=255)
                                                                                                              tggtggTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGAAAGAGCTGGCGAACGTGCAGGa  <  2:671481/139‑1 (MQ=255)
                                                                                                                      tcCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAAc                                                                     <  2:264280/64‑1 (MQ=255)
                                                                                                                      tcCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAAc                                                                     >  1:264280/1‑64 (MQ=255)
                                                                                                                          |                                                                                                                              
ACTACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGAAAGAGCTGGCGAACGTGCAGGA  >  NC_000913/3936259‑3936507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: