BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,971,307	G→A	L16L (CTG→CTA)	wecC →	UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,971,307	0	G	A	100.0%	144.1 / NA	41	L16L (CTG→CTA)	wecC	UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/20); total (21/20)

TATGGTGATGGTCAGGCATGCTCTCGCATTCTGGAAGCGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTGCCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGCGAGTG  >  NC_000913/3971198‑3971428

tATGGTGATGGTCAGGCATGCTCTCGCATTCTGGAAGCGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCa                                                                                              >  1:401356/1‑139 (MQ=255)

    gTGATGGTCAGGCATGCTCTCGCATTCTGGAAGCGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCaaaaa                                                                                          <  1:634676/139‑1 (MQ=255)

            cAGGCATGCTCTCGCATTTTGGAAGCGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAAt                                                                                  >  1:387649/1‑139 (MQ=255)

            cAGGCATGCTCTCGCATTCTGGAAGCGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAAt                                                                                  <  2:382988/139‑1 (MQ=255)

                    ctctCGCATTCTGGAAGCGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTACCTCACGGCAAAAACAGGTAATTGGTGTCg                                                                          >  2:374637/1‑139 (MQ=255)

                               tGGAAGCGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCaa                                                                                             <  2:294962/109‑1 (MQ=255)

                               tGGAAGCGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCaa                                                                                             >  1:294962/1‑109 (MQ=255)

                               tGGAAGCGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGACaa                                                                                             >  1:125191/1‑109 (MQ=255)

                               tGGAAGCGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGACaa                                                                                             <  2:125191/109‑1 (MQ=255)

                                   aGCGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATg                                                           <  2:387649/139‑1 (MQ=255)

                                     cGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATg                                                           <  2:557664/137‑1 (MQ=255)

                                     cGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATg                                                           >  1:557664/1‑137 (MQ=255)

                                       ttAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACGAACGGCAGCAGCGTTTGCCTCACGGCaaaaa                                                                                          >  2:424216/1‑104 (MQ=255)

                                       ttAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCaaaaa                                                                                          <  1:424216/104‑1 (MQ=255)

                                         aaaaaaTAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTACCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTg                                                     <  1:374637/139‑1 (MQ=255)

                                                 atCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATc                                             >  2:588723/1‑139 (MQ=255)

                                                 atCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATc                                             >  2:115623/1‑139 (MQ=255)

                                                  tCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCa                                            >  2:198483/1‑139 (MQ=255)

                                                               tGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCg                                   <  2:400981/135‑1 (MQ=255)

                                                               tGAGTTTTGCGACCATTGCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCg                                   >  1:400981/1‑135 (MQ=255)

                                                                   ttttGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCat                           <  1:115623/139‑1 (MQ=255)

                                                                    tttGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACAt                                                            <  1:386585/105‑1 (MQ=255)

                                                                    tttGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACAt                                                            >  2:386585/1‑105 (MQ=255)

                                                                      tGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGa                                                                         >  1:481408/1‑90 (MQ=255)

                                                                      tGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGa                                                                         <  2:481408/90‑1 (MQ=255)

                                                                        cGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATAtcgt                      <  1:588723/139‑1 (MQ=255)

                                                                          aCCATTTCTGTTATCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATAtcgtcg                    >  1:256899/1‑139 (MQ=255)

                                                                                       tCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGAt                                                   <  1:526844/95‑1 (MQ=255)

                                                                                       tCGGACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGAt                                                   >  2:526844/1‑95 (MQ=255)

                                                                                         ggACTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGCGa     <  2:401356/139‑1 (MQ=255)

                                                                                            cTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGata                                                                       <  1:407675/70‑1 (MQ=255)

                                                                                            cTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGata                                                                       >  2:407675/1‑70 (MQ=255)

                                                                                            cTGGGTTATATCGGGCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGCGAgtg  >  2:186568/1‑139 (MQ=255)

                                                                                                         ggCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGgtg                                                                             <  2:303402/51‑1 (MQ=255)

                                                                                                         ggCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGgtg                                                                             >  1:303402/1‑51 (MQ=255)

                                                                                                         ggCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGa                                                                         <  1:461508/55‑1 (MQ=255)

                                                                                                         ggCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGa                                                                         >  2:461508/1‑55 (MQ=255)

                                                                                                         ggCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGc       >  2:213117/1‑121 (MQ=255)

                                                                                                         ggCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGc       <  1:7553/121‑1 (MQ=255)

                                                                                                         ggCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGc       <  1:213117/121‑1 (MQ=255)

                                                                                                         ggCTACCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGc       >  2:7553/1‑121 (MQ=255)

TATGGTGATGGTCAGGCATGCTCTCGCATTCTGGAAGCGTTAAAAAATAATCGGATATCACTATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTATATCGGGCTGCCAACGGCAGCAGCGTTTGCCTCACGGCAAAAACAGGTAATTGGTGTCGATATCAACCAACATGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATTTGGCGAGTG  >  NC_000913/3971198‑3971428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`