Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,182,907 T→C Y555H (TAC→CAC)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,182,9070TC100.0% 116.9 / NA 31Y555H (TAC→CAC) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/17);  total (14/17)

CTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGAC  >  NC_000913/4182775‑4183032
                                                                                                                                    |                                                                                                                             
cTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTc                                                                                                                         >  2:664653/1‑139 (MQ=255)
                   aGAACAACCCGCTGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGCACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAAc                                                                                                      <  2:486585/139‑1 (MQ=255)
                     aacaacCCGCTGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAAccc                                                                                                    >  2:601647/1‑139 (MQ=255)
                         acCCGCTGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGa                                                                                                <  2:224440/139‑1 (MQ=255)
                               tGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGa                                                                                                         <  1:441061/124‑1 (MQ=255)
                               tGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGa                                                                                                         >  2:441061/1‑124 (MQ=255)
                               tGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc                                                                                          >  1:133439/1‑139 (MQ=255)
                                      gaTTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATc                                                                                   <  2:216818/139‑1 (MQ=255)
                                               caAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGATGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATc                                                                          <  1:428547/139‑1 (MQ=255)
                                                      cgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActctc                                                                   >  2:495846/1‑139 (MQ=255)
                                                          tctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTc                                                               <  2:201316/139‑1 (MQ=255)
                                                            tcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCg                                                             >  1:283589/1‑139 (MQ=255)
                                                                    cGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca                                                     <  1:611507/139‑1 (MQ=255)
                                                                     ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                                                                                  <  1:316728/109‑1 (MQ=255)
                                                                     ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg                                                                                  >  2:316728/1‑109 (MQ=255)
                                                                     ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGcac                                                    >  2:321709/1‑139 (MQ=255)
                                                                      gCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACAt                                                                                    <  1:507348/106‑1 (MQ=255)
                                                                      gCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACAt                                                                                    >  2:507348/1‑106 (MQ=255)
                                                                                 cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt                                                                              >  2:651236/1‑101 (MQ=255)
                                                                                 cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt                                                                              <  1:651236/101‑1 (MQ=255)
                                                                                     cccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActct                                                                    <  1:648962/107‑1 (MQ=255)
                                                                                     cccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActct                                                                    >  2:648962/1‑107 (MQ=255)
                                                                                        gTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt                                 <  1:321709/139‑1 (MQ=255)
                                                                                         tGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTAt                                                                                                                   <  1:606164/56‑1 (MQ=255)
                                                                                         tGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTAt                                                                                                                   >  2:606164/1‑56 (MQ=255)
                                                                                             cGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTg                            <  1:664653/139‑1 (MQ=255)
                                                                                                  aGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt                       <  2:133439/139‑1 (MQ=255)
                                                                                                   ggCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc                                   <  2:33770/126‑1 (MQ=255)
                                                                                                   ggCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc                                   >  1:33770/1‑126 (MQ=255)
                                                                                                              tCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg          >  2:486969/1‑139 (MQ=255)
                                                                                                                       acacCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGAc  <  1:601647/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                             
CTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGAC  >  NC_000913/4182775‑4183032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: