Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,814,8270TC37.5% 5.5 / 14.3 16noncoding (26/78 nt)micAsRNA antisense regulator of ompA, lamB, ompX, and phoP, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base T (6/4);  new base C (3/3);  total (9/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGC  >  NC_000913/2814712‑2814965
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tgAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATTCAGAGATGAAAttt                                                                                                                     >  1:219835/1‑139 (MQ=255)
 gAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAAtttt                                                                                                                    >  1:269352/1‑139 (MQ=255)
    gCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGc                                                                                                                 <  2:151798/139‑1 (MQ=255)
               acGGCATTTAGCCACCTCCGGTAATTTTTGTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGt                                                                                                      >  2:74740/1‑139 (MQ=255)
                                                              ctttcttCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCACCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCaaa                                                       <  2:219835/139‑1 (MQ=255)
                                                                         ggCGAGTCTGTGTATATGAAAGACGCGCATATGTTAGCATCATCCCTGAATTCAGAGATGTAATTTTGGCCACTCACGAGAGGCCTTTTTCTTTTCTGTCAGGCGTGTATTTCAAGCCACACCGCAAACGGTGCGGTAt                                            >  1:176038/1‑139 (MQ=255)
                                                                               tCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCgg                                      >  2:232158/1‑139 (MQ=255)
                                                                               acTGAGTATATGAAAGCCGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCgg                                      >  2:580123/2‑139 (MQ=255)
                                                                                             aGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCctgc                        >  1:94687/1‑139 (MQ=255)
                                                                                               aCGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCaaa                                                       >  1:578572/1‑106 (MQ=255)
                                                                                               aCGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCaaa                                                       <  2:578572/106‑1 (MQ=255)
                                                                                               aCGCGCATTTGTTATCATCACCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAAc                                                      <  1:494802/107‑1 (MQ=255)
                                                                                               aCGCGCATTTGTTATCATCACCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAAc                                                      >  2:494802/1‑107 (MQ=255)
                                                                                                   gcATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACgg                  <  1:580123/139‑1 (MQ=255)
                                                                                                   gcATTTGTTATCATCACCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACgg                  <  1:598954/139‑1 (MQ=255)
                                                                                                         gTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCa            <  1:232158/139‑1 (MQ=255)
                                                                                                                   tcCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGCTCGGTATCAGCGGCATCCATTTCCTGCTGACGGCGTTCAGACGCCTcgc  >  2:455304/1‑139 (MQ=255)
                                                                                                                   |                                                                                                                                          
TGAAGCTATCTAACAACGGCATTTAGCCACCTCCGGTAATTTTTTTAAAAATTTTCTGAACTCTTTCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGC  >  NC_000913/2814712‑2814965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: