Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,093,4630TC71.9% 49.9 / 19.4 32V196V (GTT→GTC) ycdTdiguanylate cyclase, membrane‑anchored
Reads supporting (aligned to +/- strand):  ref base T (4/5);  new base C (11/12);  total (15/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-02
Rejected as consensus: Frequency below/above cutoff threshold.

TTTTTGTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATCTCTCTGTTATTTATGAG  >  NC_000913/1093342‑1093600
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tttttgtttttgGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCAt                                                                                                                          <  2:425109/139‑1 (MQ=255)
     gtttttgGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGt                                                                                                                     <  2:238564/139‑1 (MQ=255)
                gCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCtt                                                                                                          >  1:24578/1‑139 (MQ=255)
                    aTAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCgg                                                                                                      <  2:590558/139‑1 (MQ=255)
                     tAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGt                                                                                                     <  1:420468/139‑1 (MQ=255)
                           cTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCt                                                                                               >  2:425921/1‑139 (MQ=255)
                            tCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCta                                                                                              <  2:151826/139‑1 (MQ=255)
                                        tAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGt                                                                                  >  1:215982/1‑139 (MQ=255)
                                          gTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGt                                                                                  <  1:22507/137‑1 (MQ=255)
                                          gTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGt                                                                                  >  2:22507/1‑137 (MQ=255)
                                          gTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGttt                                                                                >  1:483406/1‑139 (MQ=255)
                                                       cTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTATCCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATc                                                                   >  1:77718/1‑139 (MQ=255)
                                                                    aTTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGa                                                      >  1:40079/1‑139 (MQ=255)
                                                                     ttGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGAc                                                     >  1:563155/1‑139 (MQ=255)
                                                                     ctGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGAc                                                     >  1:384085/2‑139 (MQ=255)
                                                                      tGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTaa                                                                                      >  2:61517/1‑105 (MQ=255)
                                                                      tGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTaa                                                                                      <  1:61517/105‑1 (MQ=255)
                                                                      tGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGatg                                                    >  1:512693/1‑137 (MQ=255)
                                                                                aCCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCtt                                          >  2:173462/1‑139 (MQ=255)
                                                                                 ccAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCttt                                         <  2:24578/139‑1 (MQ=255)
                                                                                      gAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTtgtg                                    <  1:425921/139‑1 (MQ=255)
                                                                                      gAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTtgtg                                    <  2:512693/139‑1 (MQ=255)
                                                                                         aaTGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCg                                 <  2:563155/139‑1 (MQ=255)
                                                                                           tGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTa                               <  2:215982/139‑1 (MQ=255)
                                                                                           tGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTa                               <  1:502050/139‑1 (MQ=255)
                                                                                           tGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTa                               <  1:173462/139‑1 (MQ=255)
                                                                                           tGGTCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTa                               <  2:483406/139‑1 (MQ=255)
                                                                                              tCAGGTGGTTTGGAAAGCCTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTg                            <  1:531543/139‑1 (MQ=255)
                                                                                                               ccTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCt                                                                                                           >  1:283210/1‑43 (MQ=255)
                                                                                                               ccTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCt                                                                                                           <  2:283210/43‑1 (MQ=255)
                                                                                                                cTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATCTCTCTGtta          >  2:554309/1‑139 (MQ=255)
                                                                                                                cTCATATGTCACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATCTCTCTGtta          >  2:243385/1‑139 (MQ=255)
                                                                                                                        ttACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATCTCTCTGTTATTTATGAg  <  1:215292/139‑1 (MQ=255)
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TTTTTGTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATCTCTCTGTTATTTATGAG  >  NC_000913/1093342‑1093600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑