Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,684,175 T→C S432P (TCT→CCT)  rstB → sensory histidine kinase of RstAB two‑component system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,684,1750TC96.7% 106.1 / ‑2.7 30S432P (TCT→CCT) rstBsensory histidine kinase of RstAB two‑component system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (14/15);  minor base A (1/0);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

GGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCG  >  NC_000913/1684049‑1684302
                                                                                                                              |                                                                                                                               
gggctggCAATTGTCCACTCTATAGCACTGGCAATGGGTGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACt                                                                                                                     <  2:76308/139‑1 (MQ=255)
      gCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACgcgc                                                                                                               >  1:454151/1‑139 (MQ=255)
              ccACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGt                                                                                                       <  1:83697/139‑1 (MQ=255)
                 ctctATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCaa                                                                                                    <  1:524836/139‑1 (MQ=255)
                      taGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACGCTACGCGCACGATGGTCAAGTcac                                                                                               >  1:426609/1‑139 (MQ=255)
                            tGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCaa                                                                                                    <  2:269736/128‑1 (MQ=255)
                            tGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCaa                                                                                                    >  1:269736/1‑128 (MQ=255)
                            tGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGAc                                                                                         >  2:203974/1‑139 (MQ=255)
                            tGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGAc                                                                                         >  1:479910/1‑139 (MQ=255)
                             ggCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACt                                                                                        <  2:116641/139‑1 (MQ=255)
                              gCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGt                                                                                                       <  1:102657/123‑1 (MQ=255)
                              gCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGt                                                                                                       >  2:102657/1‑123 (MQ=255)
                              gCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACtg                                                                                       >  1:210911/1‑139 (MQ=255)
                              gCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACtg                                                                                       >  2:444744/1‑139 (MQ=255)
                                             gTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACGCTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGt                                                                        <  2:426609/139‑1 (MQ=255)
                                             gTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGt                                                                        <  2:479910/139‑1 (MQ=255)
                                             gTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGt                                                                        <  2:117878/139‑1 (MQ=255)
                                                            aGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTATGTTGTAACTAAAg                                                         <  2:454151/139‑1 (MQ=255)
                                                             gCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCtat                                                                                 <  1:315806/114‑1 (MQ=255)
                                                             gCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCtat                                                                                 >  2:315806/1‑114 (MQ=255)
                                                              cGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAGCATCCCGCAATTTACccct                                                                                                                               >  1:22748/1‑67 (MQ=255)
                                                              cGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAGCATCCCGCAATTTACccct                                                                                                                               <  2:22748/67‑1 (MQ=255)
                                                                  cTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTATGTTGTAACTAAAGTGGTTa                                                   <  2:345991/139‑1 (MQ=255)
                                                                     ggtggtGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTATGTTGTAACTAAAGTGGTTAata                                                >  1:79646/1‑139 (MQ=255)
                                                                            cccGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTATGTTGTAACTAAAGTGGTTAATATTATGgc                                         >  2:589836/1‑139 (MQ=255)
                                                                             ccGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCACTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTATGTTGTAACTAAAGTGGTTAATATTATGgcg                                        >  2:279729/1‑139 (MQ=255)
                                                                                    tcGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACg                                 <  1:203974/139‑1 (MQ=255)
                                                                                              ggCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACtgtg                                                                                     >  1:87125/1‑77 (MQ=255)
                                                                                              ggCCGTTATGGCATAACATCCCGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACtgtg                                                                                     <  2:87125/77‑1 (MQ=255)
                                                                                                                   cGCAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCg  >  1:23797/1‑139 (MQ=255)
                                                                                                                     cAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTAtgttgt                                                                 >  2:337681/1‑74 (MQ=255)
                                                                                                                     cAATTTACCCCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTAtgttgt                                                                 <  1:337681/74‑1 (MQ=255)
                                                                                                                              |                                                                                                                               
GGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTATGTTGTAACTAAAGTGGTTAATATTATGGCGCGTTACGATCTCGTAGACCGACTCAACACTACCTTTCG  >  NC_000913/1684049‑1684302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: