Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,071,304	A→G	intergenic (+93/‑13)	yeeP → / → isrC	pseudogene, CP4‑44 prophage, 50S ribosome‑binding GTPase family,Phage or Prophage Related, putative histone/novel sRNA, CP4‑44, putative prophage remnant

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,071,304	0	A	G	100.0%	189.7 / NA	50	intergenic (+93/‑13)	yeeP/isrC	pseudogene, CP4‑44 prophage, 50S ribosome‑binding GTPase family,Phage or Prophage Related, putative histone/novel sRNA, CP4‑44, putative prophage remnant
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/27);  total (23/27)

CTCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAACAACAGAACCACAATTCAGAAA  >  NC_000913/2071172‑2071438                                                                                                                                     |                                                                                                                                       ctctGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGaa                                                                                                                                  >  1:330786/1‑139 (MQ=255)    tGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATaa                                                                                                                               >  1:188929/1‑139 (MQ=255)         cccGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGa                                                                                                                          >  1:100291/1‑139 (MQ=255)          ccGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGAt                                                                                                                         <  2:142084/139‑1 (MQ=255)          ccGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGAt                                                                                                                         <  1:168238/139‑1 (MQ=255)           cGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATc                                                                                                                        >  2:462319/1‑139 (MQ=255)              gCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAat                                                                                                                     <  2:188929/139‑1 (MQ=255)              gCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAat                                                                                                                     <  2:211226/139‑1 (MQ=255)                           ggATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        <  2:468982/139‑1 (MQ=255)                           ggATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        <  1:291570/139‑1 (MQ=255)                            gATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcga                                                                                                       <  1:187605/139‑1 (MQ=255)                            gATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        <  2:353291/138‑1 (MQ=255)                            gATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        >  1:353291/1‑138 (MQ=255)                                             gTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATAtcgatcga                                                                                      <  2:554472/139‑1 (MQ=255)                                             gTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATAtcgatcga                                                                                      >  2:120955/1‑139 (MQ=255)                                                   aCTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCt                                                                                >  1:293987/1‑139 (MQ=255)                                                       aTGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcgat                                                                                                      <  1:295871/113‑1 (MQ=255)                                                       aTGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcgat                                                                                                      >  2:295871/1‑113 (MQ=255)                                                        tGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGAt                                                                                                                         >  1:59971/1‑93 (MQ=255)                                                        tGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGAt                                                                                                                         <  2:59971/93‑1 (MQ=255)                                                        tGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        >  2:59746/1‑110 (MQ=255)                                                        tGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        <  1:59746/110‑1 (MQ=255)                                                          ccctccctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaataata                                                                         <  2:127132/139‑1 (MQ=255)                                                               cctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCtt                                                                    <  1:462319/139‑1 (MQ=255)                                                                         aGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        <  2:559158/93‑1 (MQ=255)                                                                         aGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        >  1:559158/1‑93 (MQ=255)                                                                         aGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAAc                                                          >  2:547033/1‑139 (MQ=255)                                                                             tGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATAtcgatcga                                                                                      <  2:195737/107‑1 (MQ=255)                                                                             tGCTCACAAAAGCACTGTTTTCATTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATAtcgatcga                                                                                      >  1:195737/1‑107 (MQ=255)                                                                              gCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGca                                                     <  2:330786/139‑1 (MQ=255)                                                                               cTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGAt                                                                                                                         <  1:122207/70‑1 (MQ=255)                                                                               cTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGAt                                                                                                                         >  2:122207/1‑70 (MQ=255)                                                                               cTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        <  2:257938/87‑1 (MQ=255)                                                                               cTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        >  1:257938/1‑87 (MQ=255)                                                                                 cacaAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAg                                                  >  1:613781/1‑139 (MQ=255)                                                                                   caAAAGGACTGTTTTAGTTACTGATTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATCTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGAt                                                <  1:224387/139‑1 (MQ=255)                                                                                   caAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGAt                                                <  1:547033/139‑1 (MQ=255)                                                                                                        tGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATcgatcgat                                                                                     <  2:269263/81‑1 (MQ=255)                                                                                                        tGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATcgatcgat                                                                                     >  1:269263/1‑81 (MQ=255)                                                                                                         gTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAggg                          <  2:100291/139‑1 (MQ=255)                                                                                                          tctctcTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        >  2:68728/1‑60 (MQ=255)                                                                                                          tctctcTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        <  1:68728/60‑1 (MQ=255)                                                                                                                 tGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAACAACAg                  <  2:613781/139‑1 (MQ=255)                                                                                                                    ccGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCa                                                               >  2:3377/1‑91 (MQ=255)                                                                                                                    ccGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCa                                                               <  1:3377/91‑1 (MQ=255)                                                                                                                     cGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAACAACAGAAcc              >  1:407458/1‑139 (MQ=255)                                                                                                                      gTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGt                                                                 >  2:460267/1‑87 (MQ=255)                                                                                                                      gTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGt                                                                 <  1:460267/87‑1 (MQ=255)                                                                                                                      gTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAACAAAAGAACca             >  1:228703/1‑139 (MQ=255)                                                                                                                                 cAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAACAACAGAACCACAATTCAGaaa  >  1:176625/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                       CTCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAACAACAGAACCACAATTCAGAAA  >  NC_000913/2071172‑2071438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑