| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 245,803 | C→T | M1M (ATG→ATA) † | yafK ← | L,D‑transpeptidase‑related protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 245,803 | 0 | C | T | 75.0% | 48.0 / 17.7 | 32 | M1M (ATG→ATA) † | yafK | L,D‑transpeptidase‑related protein |
| Reads supporting (aligned to +/- strand): ref base C (4/4); new base T (14/10); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
GACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTCACATTATCACCAAAAGAGTTTTACGCAAG > NC_000913/245674‑245919 | gACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTACtt > 1:42739/1‑139 (MQ=255) gggATCCTATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTcc > 1:71660/1‑139 (MQ=255) cAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTc < 1:418813/128‑1 (MQ=255) cAAATGCTGCTTATACTCTTCGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCGTATTc > 2:418813/1‑128 (MQ=255) ctgctTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTac > 2:448290/1‑139 (MQ=255) tgctTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTaca < 2:151132/139‑1 (MQ=255) gctTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCAt > 2:187990/1‑109 (MQ=255) gctTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCAt < 1:187990/109‑1 (MQ=255) ctTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGa < 2:71660/139‑1 (MQ=255) ttGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACg > 2:523578/1‑139 (MQ=255) tGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACgg > 1:336830/1‑139 (MQ=255) caGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACGTCGCAAGAATTAATACGAATTTACGTATACTGCTTATTCCTTGATaaa > 2:159882/1‑111 (MQ=255) caGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATaaa < 1:159882/111‑1 (MQ=255) cGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCCAGAATTTATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTaca < 1:355766/116‑1 (MQ=255) cGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGAt > 2:322101/1‑139 (MQ=255) cGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTaca > 2:355766/1‑116 (MQ=255) tGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATcc > 2:422777/1‑139 (MQ=255) gagCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCaaaa < 2:129871/127‑1 (MQ=255) gagCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCaaaa > 1:129871/1‑127 (MQ=255) aCTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGaa < 1:6032/139‑1 (MQ=255) gctgcCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTaca > 1:151401/1‑101 (MQ=255) gctgcCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTaca < 2:151401/101‑1 (MQ=255) ctgcCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGt < 1:448290/139‑1 (MQ=255) cAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCt < 1:523578/139‑1 (MQ=255) aCGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATcc < 2:253280/97‑1 (MQ=255) aCGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATcc > 1:253280/1‑97 (MQ=255) gggATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTCACATTATCACCaa > 1:558415/1‑139 (MQ=255) gATCAACAACATCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTCACATTATCACCaaaa > 2:456830/1‑139 (MQ=255) acaacaTCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTCACATTATCACCAAAAGAGtt < 1:422777/139‑1 (MQ=255) aTCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTaca < 1:94025/58‑1 (MQ=255) aTCGCAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTaca > 2:94025/1‑58 (MQ=255) cAAGAATTAATGCGATTTTACGTATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTCACATTATCACCAAAAGAGTTTTACGCAAg > 2:89857/1‑139 (MQ=255) | GACAGGGGATCCCATCAACTGCTGCTTATACTCTTTGCTCACAGGCGTGGTAGAGCTACTGCTGCCCAGCAAACCGGCAAATGAAACGCACGGGATCAACAACATCGCAAGAATTAATGCGATTTTACGCATACTGCTTATTCCTTGATAAAACGGTTACACACGCCAGGACGGCAAAATGGATCCCAAATCGGAATAGTCTGGATTTGGAAGGCTCACATTATCACCAAAAGAGTTTTACGCAAG > NC_000913/245674‑245919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |