Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,092,066	G→A	G324S (GGC→AGC)	hisD →	bifunctional histidinal dehydrogenase/ histidinol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,092,066	0	G	A	100.0%	68.7 / NA	21	G324S (GGC→AGC)	hisD	bifunctional histidinal dehydrogenase/ histidinol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/11);  total (10/11)

TCGCGTTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAATCGGCAGGTGATTACGCCTCCGGCACCAACCACGTTC  >  NC_000913/2091933‑2092201                                                                                                                                      |                                                                                                                                        tcgcgtTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCg                                                                                                                                    >  2:609379/1‑139 (MQ=255)  cgcgtTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCgg                                                                                                                                   <  1:480764/139‑1 (MQ=255)  cgcgtTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCgg                                                                                                                                   <  1:618567/139‑1 (MQ=255)    cgtTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAg                                                                                                                                 <  2:37208/139‑1 (MQ=255)                cGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCAtt                                                                                                                     <  1:609379/139‑1 (MQ=255)                 gTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCa                                                                                                                               <  1:183660/128‑1 (MQ=255)                 gTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCa                                                                                                                               >  2:183660/1‑128 (MQ=255)                                                                 gCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGtt                                                                    <  2:248287/139‑1 (MQ=255)                                                                       aaCGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCgg                                                                      <  2:560942/131‑1 (MQ=255)                                                                       aaCGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCgg                                                                      >  1:560942/1‑131 (MQ=255)                                                                                    tGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTg                                                 <  2:171097/139‑1 (MQ=255)                                                                                                 aGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCg                                                                                         <  2:540497/86‑1 (MQ=255)                                                                                                 aGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCg                                                                                         >  1:540497/1‑86 (MQ=255)                                                                                                        gcgcAGTGCGTGGAGATCTCCAACCAGTACAGCCCg                                                                                                                                    >  2:97227/1‑36 (MQ=255)                                                                                                        gcgcAGTGCGTGGAGATCTCCAACCAGTACAGCCCg                                                                                                                                    <  1:97227/36‑1 (MQ=255)                                                                                                             gTGCGTGGAGATCTCCAACCAGTACAGCCCGGAGCACCAGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGAt                                                                                       <  2:411931/76‑1 (MQ=255)                                                                                                             gTGCGTGGAGATCTCAAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGAt                                                                                       >  1:411931/1‑76 (MQ=255)                                                                                                                        tctcCAACCAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAATCGGCAGGTGATTACGCCTCCGGCac             >  1:535995/1‑139 (MQ=255)                                                                                                                               ccAGTACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAATCGGCAGGTGATTACGCCTCCGGCACCAACCAc      >  2:334612/1‑139 (MQ=255)                                                                                                                                   tACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAATCGGCAGGTGATTACGCCTCCGGCACCAACCACGTTc  >  1:424966/1‑139 (MQ=255)                                                                                                                                   tACAGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAATCGGCAGGTGATTACGCCTCCGGCACCAACCACGTTc  >  1:136383/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                        TCGCGTTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAATCGGCAGGTGATTACGCCTCCGGCACCAACCACGTTC  >  NC_000913/2091933‑2092201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑