Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,505,003	T→C	H182R (CAC→CGC)	ypdE ←	aminopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,505,003	0	T	C	100.0%	49.7 / NA	14	H182R (CAC→CGC)	ypdE	aminopeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/6);  total (8/6)

TCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATT  >  NC_000913/2504869‑2505131                                                                                                                                       |                                                                                                                                 tCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAg                                                                                                                              >  2:457304/1‑139 (MQ=255)         cagGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCa                                                                                                                      <  1:240949/139‑1 (MQ=255)              ggTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAAc                                                                                                                 <  2:302056/139‑1 (MQ=255)                            tGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATa                                                                                                   >  1:458556/1‑139 (MQ=255)                                         cGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCgg                                                                                      <  1:302429/139‑1 (MQ=255)                                                   cgGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAgg                                                                            <  1:317324/139‑1 (MQ=255)                                                                                                  tGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCa                             >  2:502928/1‑139 (MQ=255)                                                                                                               acTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTg                                   <  1:144340/120‑1 (MQ=255)                                                                                                               acTTCCGCAGGTAGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTg                                   >  2:144340/1‑120 (MQ=255)                                                                                                                           aGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGc                                                           <  1:393106/84‑1 (MQ=255)                                                                                                                           aGTTCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGc                                                           >  2:393106/1‑84 (MQ=255)                                                                                                                             ttCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATTAAACGTGACGCGATCGCCGGGACGAAtt  >  2:565673/1‑139 (MQ=255)                                                                                                                             ttCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGTCGGGACGAAtt  >  2:400669/1‑139 (MQ=255)                                                                                                                             ttCGGCGTCGCGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAAtt  >  1:49557/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                 TCGCCCAGCAGGCGGTATCAAGCACAATGGCGACGTCCGGCGACACCGCGCGGGTGGCAGTTTGCCCGCCGCGTAATCCCACCTCTTCGCTGGAACTTGCCACCAGCCACACTTCCGCAGGTAGTTCGGCGTCGTGCAGTTCGCGCAGTAACGTCACCAGCAGATAGCAACCGAGGCGGTCATCAAAGGCTTTCCCCATCACTCGCTGGTGAGGGAGAACCTGAAAAGTGGTATCAAACGTGACGCGATCGCCGGGACGAATT  >  NC_000913/2504869‑2505131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑