Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,706,066	T→C	E353E (GAA→GAG)	lepA ←	back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,706,066	0	T	C	100.0%	77.2 / NA	21	E353E (GAA→GAG)	lepA	back‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/10);  total (11/10)

GGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGA  >  NC_000913/2705953‑2706196                                                                                                                  |                                                                                                                                   ggcagCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTgcagca                                                                                                           >  1:352279/1‑139 (MQ=255)          gATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGa                                                                                                  <  1:246049/139‑1 (MQ=255)                      aCGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGa                                                                                      <  2:352279/139‑1 (MQ=255)                                                  cAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTc                                                          <  2:566993/139‑1 (MQ=255)                                                      ttCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGc                                                      <  1:371954/139‑1 (MQ=255)                                                               tACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                        >  2:571254/1‑128 (MQ=255)                                                               tACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                        <  1:571254/128‑1 (MQ=255)                                                                 cGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                        <  2:530212/126‑1 (MQ=255)                                                                 cGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCg                                                        >  1:530212/1‑126 (MQ=255)                                                                 cGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAg                                           >  1:103078/1‑139 (MQ=255)                                                                  ggTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGt                                          >  2:110786/1‑139 (MQ=255)                                                                    tCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGa                                        >  2:116816/1‑139 (MQ=255)                                                                       gcgcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGc                                     >  2:134700/1‑139 (MQ=255)                                                                                  aTCAGATCCAGATCGTATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGc                          <  1:110786/139‑1 (MQ=255)                                                                                                 tATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGTAGCCGCAGCGGAAACCAAAGCACAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAgcg           >  2:377414/1‑139 (MQ=255)                                                                                                 tATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAgcg           >  1:462442/1‑139 (MQ=255)                                                                                                 tATTCACGTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAgcg           >  2:443327/1‑139 (MQ=255)                                                                                                        gTTCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACg    <  1:134700/139‑1 (MQ=255)                                                                                                          tCCAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGa  <  2:462442/139‑1 (MQ=255)                                                                                                           ccAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGcgcg         <  2:135795/131‑1 (MQ=255)                                                                                                           ccAGACGCTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGcgcg         >  1:135795/1‑131 (MQ=255)                                                                                                                  |                                                                                                                                   GGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGA  >  NC_000913/2705953‑2706196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑