Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,744,303	A→G	T76T (ACT→ACC)	rplS ←	50S ribosomal subunit protein L19

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,744,303	0	A	G	100.0%	62.9 / NA	18	T76T (ACT→ACC)	rplS	50S ribosomal subunit protein L19
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/7);  total (11/7)

GAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCTTCAACAA  >  NC_000913/2744177‑2744436                                                                                                                               |                                                                                                                                      gAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGaca                                                                                                                           <  2:16150/139‑1 (MQ=255)                              gAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAAtttt                                                                                              >  2:566355/1‑139 (MQ=255)                                gcagcCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTAc                                                                                            >  2:65153/1‑139 (MQ=255)                                        aCCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTg                                                                                    >  2:483588/1‑139 (MQ=255)                                              aCGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCa                                                                              <  1:566355/139‑1 (MQ=255)                                                        aGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGAc                                                                    <  1:418469/139‑1 (MQ=255)                                                                   tttAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGa                                                         <  1:117175/139‑1 (MQ=255)                                                                   tttAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGa                                                         >  2:117175/1‑139 (MQ=255)                                                                    ttAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGaca                                                                                                                           <  2:164181/72‑1 (MQ=255)                                                                    ttAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGaca                                                                                                                           >  1:164181/1‑72 (MQ=255)                                                                    ttAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGaa                                                        >  1:394861/1‑139 (MQ=255)                                                                    ttAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGaa                                                        <  1:65153/139‑1 (MQ=255)                                                                    ttAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGaa                                                        >  2:206400/1‑139 (MQ=255)                                                                     tAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAAt                                                       >  1:212829/1‑139 (MQ=255)                                                                                aCAGCACCATGACGTTTGACAGAAATGCTGTCAATTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCa                                            <  2:394861/139‑1 (MQ=255)                                                                                   gCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGc                                         >  1:493815/1‑139 (MQ=255)                                                                                                 gACAGAAATGCTGTCAACTACCGGAGACTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCATCGAATGCCTGc                           >  1:367499/1‑139 (MQ=255)                                                                                                                    aCCGGAGAGTGGGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCtt        >  1:558865/1‑139 (MQ=255)                                                                                                                          gagtgggtCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCTTcaacaa  >  1:373295/1‑139 (MQ=255)                                                                                                                           agtgggtCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGAc                                                                    <  1:455272/72‑1 (MQ=255)                                                                                                                           agtgggtCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGAc                                                                    >  2:455272/1‑72 (MQ=255)                                                                                                                               |                                                                                                                                      GAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTTGGAACCTTCAACAA  >  NC_000913/2744177‑2744436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑