| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 350,431 | G→A | L140L (CTG→CTA) | prpC → | 2‑methylcitrate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 350,431 | 0 | G | A | 100.0% | 55.1 / NA | 17 | L140L (CTG→CTA) | prpC | 2‑methylcitrate synthase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/7); total (10/7) | |||||||||||
CCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCATCGCAAAG > NC_000913/350312‑350565 | ccGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATtc < 1:530263/139‑1 (MQ=255) tgtTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGt > 1:246930/1‑113 (MQ=255) tgtTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGt < 2:246930/113‑1 (MQ=255) ccAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACt > 1:541812/1‑139 (MQ=255) gCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCAc > 1:175493/1‑139 (MQ=255) cATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACt > 2:505587/1‑139 (MQ=255) cGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAg > 2:77667/1‑48 (MQ=255) cGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTAGCGTCGCTTAg < 1:77667/48‑1 (MQ=255) gcgcgcGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCa < 1:506029/87‑1 (MQ=255) gcgcgcGGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCa > 2:506029/1‑87 (MQ=255) cgcgcgGGATATTGCCGACAAACTGCTAGCGTCGCt > 1:507952/1‑36 (MQ=255) cgcgcgGGATATTGCCGACAAACTGCTAGCGTCGCt < 2:507952/36‑1 (MQ=255) cgcgGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATCTGCTGCa > 2:524607/1‑139 (MQ=255) cgGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCgg < 2:16912/91‑1 (MQ=255) cgGGATATTGCCGACAAACTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCgg > 1:16912/1‑91 (MQ=255) aaaCTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGaa < 2:332499/63‑1 (MQ=255) aaaCTGCTAGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGaa > 1:332499/1‑63 (MQ=255) tgctaGCGTCGCTTAGCTCGATTCTCCTTTATCGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCATCGCAAAg < 1:524607/139‑1 (MQ=255) | CCGGCGGCGTCGCACCCGATGGATGTTATGCGCACCGGTGTTTCCGCGCTCGGCTGCACGCTGCCAGAAAAAGAGGGGCATACCGTCTCTGGCGCGCGGGATATTGCCGACAAACTGCTGGCGTCGCTTAGCTCGATTCTCCTTTATTGGTATCACTACAGCCACAACGGCGAACGCATCCAACCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCATCGCAAAG > NC_000913/350312‑350565 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |